Local surface modification at precise position using a chemical pen

Push-pull cannula system, which was first proposed by Gaddum, has grown to be an important method for the perfusion of brain and region-selective surface treatment. However, reported push-pull cannula systems only concerned on single reagent applications. Microfluidic system was then an exciting tool for multi-reagent treatment on substrate in closed microchannels. Nowadays, it is still a challenge to apply online mixing and reaction for surface pattern in an open environment. Here, we present a novel method using a chemical pen that enables region-selective online chemical reactions for the micro-surface modification/patterning. We utilized this method to fabricate labeling protein array using an online labeling strategy. Moreover, the device was applied for local modification of biomaterials surface by using a three-component reaction at precise position. This tool was the first demonstration of design to perform online reaction of two different reagents on a real solid sample in an open environment. It was demonstrated a useful method for protein array fabrication with online labeled protein.