Back to Search
Start Over
Passive targeting of thermosensitive diblock copolymer micelles to the lungs: synthesis and characterization of poly(N-isopropylacrylamide)-block-poly(ε-caprolactone)
- Source :
- Journal of Nanobiotechnology
- Publication Year :
- 2015
- Publisher :
- BioMed Central, 2015.
-
Abstract
- Background Amphiphilic poly(N-isopropylacrylamide)-block-poly(ε-caprolactone) (PNiPAAm-b-PCL) copolymers were synthesized by ring-opening polymerization to form thermosensitive micelles as nanocarriers for bioimaging and carboplatin delivery. Results The critical micelle concentration increased from 1.8 to 3.5 mg/l following the decrease of the PNiPAAm chain length. The copolymers revealed a lower critical solution temperature (LCST) between 33 and 40°C. The copolymers self-assembled to form spherical particles of 146–199 nm in diameter. Carboplatin in micelles exhibited a slower release at 37°C relative to that at 25°C due to the gel layer formation on the micellar shell above the LCST. The micelles containing dye or carboplatin were intravenously injected into the rats for in vivo bioimaging and drug biodistribution. The bioimaging profiles showed a significant accumulation of micelles in the lungs. The micelles could minimize the reticuloendothelial system (RES) recognition of the dye. In vivo biodistribution demonstrated an improved pulmonary accumulation of carboplatin from 2.5 to 3.4 μg/mg by the micelles as compared to the control solution. Carboplatin accumulation in the heart and kidneys was reduced after encapsulation by the micelles. Conclusion This study supports the potential of PNiPAAm-b-PCL micelles to passively target the lungs and attenuate RES uptake and possible side effects. Electronic supplementary material The online version of this article (doi:10.1186/s12951-015-0103-7) contains supplementary material, which is available to authorized users.
- Subjects :
- Adult
Male
Biodistribution
Magnetic Resonance Spectroscopy
Thermosensitivity
Neutrophils
Polyesters
Biomedical Engineering
Passive targeting
Acrylic Resins
Pharmaceutical Science
Medicine (miscellaneous)
Bioengineering
Kidney
Applied Microbiology and Biotechnology
Micelle
Lower critical solution temperature
Carboplatin
Rats, Sprague-Dawley
chemistry.chemical_compound
Lactones
Copolymer
Polymer chemistry
Spectroscopy, Fourier Transform Infrared
Animals
Humans
Tissue Distribution
Lung
Caproates
Micelles
Drug Carriers
L-Lactate Dehydrogenase
Chemistry
Research
Temperature
HEK293 Cells
Critical micelle concentration
Poly(N-isopropylacrylamide)
Biophysics
Molecular Medicine
Nanocarriers
Drug carrier
Subjects
Details
- Language :
- English
- ISSN :
- 14773155
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Nanobiotechnology
- Accession number :
- edsair.doi.dedup.....4484d6bcd8461618c7c2ace890ade855