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HCV IRES Captures an Actively Translating 80S Ribosome
- Source :
- Molecular Cell. 74:1205-1214.e8
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Translation initiation of hepatitis C virus (HCV) genomic RNA is induced by an internal ribosome entry site (IRES). Our cryoelectron microscopy (cryo-EM) analysis revealed that the HCV IRES binds to the solvent side of the 40S platform of the cap-dependently translating 80S ribosome. Furthermore, we obtained the cryo-EM structures of the HCV IRES capturing the 40S subunit of the IRES-dependently translating 80S ribosome. In the elucidated structures, the HCV IRES "body," consisting of domain III except for subdomain IIIb, binds to the 40S subunit, while the "long arm," consisting of domain II, remains flexible and does not impede the ongoing translation. Biochemical experiments revealed that the cap-dependently translating ribosome becomes a better substrate for the HCV IRES than the free ribosome. Therefore, the HCV IRES is likely to efficiently induce the translation initiation of its downstream mRNA with the captured translating ribosome as soon as the ongoing translation terminates.
- Subjects :
- Models, Molecular
Protein subunit
Hepacivirus
Internal Ribosome Entry Sites
Biology
Ribosome
03 medical and health sciences
0302 clinical medicine
Eukaryotic translation
Humans
Eukaryotic Small Ribosomal Subunit
Eukaryotic Initiation Factors
Peptide Chain Initiation, Translational
Molecular Biology
030304 developmental biology
Ribosome Subunits, Small, Eukaryotic
0303 health sciences
Messenger RNA
Binding Sites
Cryoelectron Microscopy
fungi
virus diseases
Translation (biology)
Cell Biology
Ribosome Subunits, Large, Eukaryotic
Cell biology
Internal ribosome entry site
HEK293 Cells
Host-Pathogen Interactions
Nucleic Acid Conformation
RNA, Viral
Eukaryotic Ribosome
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 10972765
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- Molecular Cell
- Accession number :
- edsair.doi.dedup.....44b58aa3d2135da20cbeb7347e04b679