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Pathogenesis of spinal cord injury induced edema and neuropathic pain: expression of multiple isoforms of wnk1
Pathogenesis of spinal cord injury induced edema and neuropathic pain: expression of multiple isoforms of wnk1
- Source :
- Annals of Neurosciences
- Publication Year :
- 2014
- Publisher :
- SAGE Publications, 2014.
-
Abstract
- Background Neuropathic pain (NP) is a common occurrence following spinal cord injury (SCI). Identification of specific molecular pathways that are involved in pain syndromes has become a major priority in current SCI research. We have investigated the role of a cation-dependent chloride transporter, Cl-regulatory protein Na+-K+-Cl- 1 (NKCC1), phosphorylation profile of NKCC1 and its specific involvement in neuropathic pain following contusion SCI (cSCI) using a rat model. Administration of the NKCC1 inhibitor bumetanide (BU) increases the mean hindpaw withdrawal latency time (WLT), thermal hyperalgesia (TH) following cSCI. These results demonstrate implication of NKCC1 co-transporter and BUin SCI-induced neuropathic pain. The with-no-lysine (K)–1 (WNK1) kinase has been shown to be an important regulator of NKCC1 phosphorylation in many systems, including nocioception. Mutations in a neuronal-specific exon of WNK1 (HSN2) was identified in patients that have hereditary sensory neuropathy type II (HSANII) also implicates WNK1 in nocioception, such that these patients have loss of perception to pain, touch and heat. In our ongoing research we proposed two studies utilizing our contusion SCI (cSCI) NP model of rat. Purpose Study 1 aimed at NKCC1 expression and activity is up-regulated following cSCI in the early edema and chronic neuropathic pain phases. Study 2 aimed at identifying the expression profile of alternatively spliced WNK1 isoforms in animals exhibiting thermal hyperalgesia (TH) following cSCI. Methods Adult male Sprague Dawley rats (275–300 g) following laminectomy received cSCI at T9 with the NYU impactor-device II by dropping 10 g weight from the height of 12.5 mm. Control rats obtained laminectomy but no impaction. Following injury, functional recovery was assessed by BBB locomotor scores on day 1, 7, 14, 21, 35, and 42 and development of thermal hyperalgesia on day 21, 28, 35, and 42 day of injury by monitoring hind paw withdraw latency time (WLT) in seconds compared with the baseline data before injury. Results Increased NKCC1 may explain observed increase in magnetic resonance imaging (MRI) T2, exhibiting NKCC1 localization in neurons. This data supports NKCC1’s role in the pathogenesis of acute and chronic phases of injury, namely spinal cord edema and chronic phase neuropathic pain. NKCC1 dependent chloride influx requires the phosphorylation at specific residues. Probing for the HSN2 exon of WNK1 reveals two key findings: i) the HSN2 exon is found in alternatively spliced neuronal isoforms found at 250 kDa and 230 kDa; ii) the 250 kDa isoform is found only in tissue that is injured. Conclusions This data implicates the NKCC1/WNK1/WNK1HSN2 involvement in post-injury response that contributes to the development of neuropathic pain. Targeting this system may have therapeutic benefit.
- Subjects :
- Pathology
medicine.medical_specialty
Spinal cord injury
HSN2
Pharmacology
Neuropathic pain
Structural Neuroscience
Pathogenesis
Edema
Thermal hyperalgesia
NKCC1
medicine
WNK1
Kinase
business.industry
General Neuroscience
medicine.disease
medicine.symptom
business
Bumetanide
Research Article
MRI
medicine.drug
Subjects
Details
- ISSN :
- 09763260 and 09727531
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Annals of Neurosciences
- Accession number :
- edsair.doi.dedup.....44da2369b9f2cb6c1f9ffe2c750298eb
- Full Text :
- https://doi.org/10.5214/ans.0972.7531.210305