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Expression of sex-steroid receptors and steroidogenic enzymes in the carotid body of adult and newborn male rats
- Source :
- Brain Research. :71-82
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- This study describes the localization and pattern of expression of estradiol and progesterone receptors as well as key enzymes for steroid synthesis (i.e. P450 side-chain-cleavage--P450scc, and P450 aromatase--P450Aro) in the carotid body (CB) and superior cervical ganglion (SCG) of adult, newborn and late fetal male rats, using immunohistochemistry, Western blot and real-time RT-PCR. Our results show a constitutive expression of the beta estradiol receptor (Erbeta) and the 80 kDa and 60 kDa progesterone receptors (PR-A and PR-C) isoforms in the CB, while in the SCG Eralpha, Erbeta, PR-A and PR-C are expressed. While P450Aro staining was negative, P450scc staining was strong both in the SCG and CB. In late fetal and newborn rats, Eralpha was not detected in the CB or SCG, but a slight staining appeared for P450 aromatase in the CB, and to a lesser extent in SCG. P450scc was strongly expressed in CB and SCG of late fetal and newborn rats. We conclude that the carotid body shows a constitutive expression of Erbeta and PR and may be able to synthesize steroids, including estradiol during late fetal life.
- Subjects :
- Male
Receptors, Steroid
medicine.medical_specialty
Superior cervical ganglion
medicine.drug_class
Blotting, Western
Receptors, Estradiol
Biology
Rats, Sprague-Dawley
Aromatase
Cytochrome P-450 Enzyme System
Internal medicine
Progesterone receptor
medicine
Animals
Cholesterol Side-Chain Cleavage Enzyme
RNA, Messenger
Receptor
Molecular Biology
Analysis of Variance
Carotid Body
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
Cholesterol side-chain cleavage enzyme
Age Factors
Gene Expression Regulation, Developmental
Sex hormone receptor
Immunohistochemistry
Rats
Blot
Endocrinology
medicine.anatomical_structure
Animals, Newborn
Estrogen
Carotid body
Neurology (clinical)
Receptors, Progesterone
hormones, hormone substitutes, and hormone antagonists
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....44ed8ba69e353ab86d9199548d208e4b