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Temporal Genetic Modifications after Controlled Cortical Impact—Understanding Traumatic Brain Injury through a Systematic Network Approach

Authors :
John Chung Che Wu
Bo Ren Jheng
Mien Cheng Chen
Chia Chou Wu
Yung Hao Wong
Kai Yun Chen
Tzu Hao Chang
Bor-Sen Chen
Hsien Yong Lai
Source :
International Journal of Molecular Sciences, Vol 17, Iss 2, p 216 (2016), International Journal of Molecular Sciences; Volume 17; Issue 2; Pages: 216, International Journal of Molecular Sciences
Publication Year :
2016
Publisher :
MDPI AG, 2016.

Abstract

Traumatic brain injury (TBI) is a primary injury caused by external physical force and also a secondary injury caused by biological processes such as metabolic, cellular, and other molecular events that eventually lead to brain cell death, tissue and nerve damage, and atrophy. It is a common disease process (as opposed to an event) that causes disabilities and high death rates. In order to treat all the repercussions of this injury, treatment becomes increasingly complex and difficult throughout the evolution of a TBI. Using high-throughput microarray data, we developed a systems biology approach to explore potential molecular mechanisms at four time points post-TBI (4, 8, 24, and 72 h), using a controlled cortical impact (CCI) model. We identified 27, 50, 48, and 59 significant proteins as network biomarkers at these four time points, respectively. We present their network structures to illustrate the protein–protein interactions (PPIs). We also identified UBC (Ubiquitin C), SUMO1, CDKN1A (cyclindependent kinase inhibitor 1A), and MYC as the core network biomarkers at the four time points, respectively. Using the functional analytical tool MetaCore™, we explored regulatory mechanisms and biological processes and conducted a statistical analysis of the four networks. The analytical results support some recent findings regarding TBI and provide additional guidance and directions for future research.

Details

Language :
English
ISSN :
14220067
Volume :
17
Issue :
2
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences
Accession number :
edsair.doi.dedup.....4502f0fd5dc3f76b153ed34f43b2feb0