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Myeloid differentiation-2 is a potential biomarker for the amplification process of allergic airway sensitization in mice
Myeloid differentiation-2 is a potential biomarker for the amplification process of allergic airway sensitization in mice
- Source :
- Allergology International, Vol 64, Iss S, Pp S37-S45 (2015)
- Publication Year :
- 2015
- Publisher :
- Elsevier, 2015.
-
Abstract
- Background Allergic sensitization is a key step in the pathogenesis of asthma. However, little is known about the molecules that are critical regulators for establishing allergic sensitization of the airway. Thus, we conducted global gene expression profiling to identify candidate genes and signaling pathways involved in house dust mite (HDM)-induced allergic sensitization in the murine airway. Methods We sensitized and challenged mice with HDM or saline as a control through the airway on days 1 and 8. We evaluated eosinophilia in bronchoalveolar lavage fluid (BALF), airway inflammation, and mucus production on days 7 and 14. We extracted total RNA from lung tissues of HDM- and saline-sensitized mice on days 7 and 14. Microarray analyses were performed to identify up-regulated genes in the lungs of HDM-sensitized mice compared to the control mice. Data analyses were performed using GeneSpring software and gene networks were generated using Ingenuity Pathways Analysis (IPA). Results We identified 50 HDM-mediated, stepwise up-regulated genes in response to allergic sensitization and amplification of allergic airway inflammation. The highest expressed gene was myeloid differentiation-2 (MD-2), a lipopolysaccharide (LPS)-binding component of Toll-like receptor (TLR) 4 signaling complex. MD-2 protein was expressed in lung vascular endothelial cells and was increased in the serum of HDM-sensitized mice, but not in the control mice. Conclusions Our data suggest MD-2 is a critical regulator of the establishment of allergic airway sensitization to HDM in mice. Serum MD-2 may represent a potential biomarker for the amplification of allergic sensitization and allergic inflammation.
- Subjects :
- Male
lcsh:Immunologic diseases. Allergy
Thymic stromal lymphopoietin
Lymphocyte Antigen 96
Sensitization
Allergic inflammation
Allergic sensitization
House dust mite
Mice
medicine
Eosinophilia
Animals
Cluster Analysis
Immunology and Allergy
Lung
biology
medicine.diagnostic_test
Gene Expression Profiling
Pyroglyphidae
Endothelial Cells
Microarray analysis
General Medicine
Allergens
respiratory system
biology.organism_classification
Asthma
respiratory tract diseases
Myeloid differentiation-2
Gene expression profiling
Toll-Like Receptor 4
Disease Models, Animal
Bronchoalveolar lavage
medicine.anatomical_structure
Gene Expression Regulation
Immunology
Immunization
medicine.symptom
lcsh:RC581-607
Biomarkers
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 13238930
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Allergology International
- Accession number :
- edsair.doi.dedup.....45313fc83e8b74a723581f9d69ec4362