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Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice
- Source :
- Immunology
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- Virus-like particles (VLPs) represent a promising vaccine against severe acute respiratory syndrome coronavirus (SARS CoV). In this study, recombinant baculovirus vAcS and vAcME were constructed to express the S protein and the M and E proteins of SARS CoV, respectively. Electron microscope analysis demonstrated the formation of VLPs in vAcME and vAcS coinfected insect cells. Mice immunized four times with VLPs developed high antibody titres against SARS CoV. In addition, VLPs elicited cell-mediated immunity as demonstrated by enhanced interferon-gamma and interleukin-4 production. VLPs also conferred protective immunity against the infection of Spike protein pseudotyped murine leukaemia virus. Our findings demonstrate that SARS CoV VLPs are immunogenic and can elicit strong SARS CoV-specific humoral and cellular immune responses in mice. This is the first study describing the immunogenicity of SARS CoV VLPs, providing valuable data for developing a protective vaccine against SARS CoV infection.
- Subjects :
- viruses
Immunology
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Antibodies, Viral
Severe Acute Respiratory Syndrome
complex mixtures
Virus
Microbiology
Interferon-gamma
Mice
Immune system
Immunity
medicine
Immunology and Allergy
Animals
Coronavirus
Immunity, Cellular
Mice, Inbred BALB C
Insect cell
biology
Immunogenicity
fungi
virus diseases
Viral Vaccines
Original Articles
biochemical phenomena, metabolism, and nutrition
Virology
Disease Models, Animal
Microscopy, Electron
Severe acute respiratory syndrome-related coronavirus
biology.protein
Female
Immunization
Severe acute respiratory syndrome coronavirus
Interleukin-4
Antibody
Subjects
Details
- ISSN :
- 13652567 and 00192805
- Volume :
- 122
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Immunology
- Accession number :
- edsair.doi.dedup.....4546c39299ecee8fc7449b8ecaca52b0
- Full Text :
- https://doi.org/10.1111/j.1365-2567.2007.02676.x