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Type III Secretion Protein, PcrV, Impairs Pseudomonas aeruginosa Biofilm Formation by Increasing M1 Macrophage-Mediated Anti-bacterial Activities
- Source :
- Frontiers in Microbiology, Frontiers in Microbiology, Vol 11 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- Pseudomonas aeruginosa biofilms employ a variety of strategies to hijack the host immune defense system to achieve chronic infection. However, the bacterial components that are involved in this process are not yet fully understood. PcrV, a needle tip protein of the P. aeruginosa type III secretion system (T3SS), was downregulated during P. aeruginosa biofilm infection. The impaired expression of the P. aeruginosa pcrV gene is associated with attenuated immune activation and an increased percentage of M2 macrophages following P. aeruginosa biofilm infection. Treatment with exogenous PcrV produced from Escherichia coli elevated tissue inflammation and the percentage of M1 macrophages, resulting in reduction in the biofilm burden. Further analyses demonstrated that the potential of PcrV to induce classically activated M1 macrophages as evidenced by the increased production of proinflammatory cytokines and anti-bacterial mediators, including inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS), as well as increased phagocytosis of bacteria. Mechanistically, PcrV-mediated promotion of macrophage M1 polarization and phagocytosis occurs through the activation of mitogen-activated protein kinases (MAPKs) and NF-κB signaling pathways. Collectively, these findings reveal a potential role of PcrV in skewing host immune defense to promote P. aeruginosa biofilm infection and provide new insights into the therapeutic strategies for P. aeruginosa biofilm infection.
- Subjects :
- Microbiology (medical)
Phagocytosis
macrophage polarization
lcsh:QR1-502
Macrophage polarization
medicine.disease_cause
Microbiology
lcsh:Microbiology
biofilm
biofilm eradication
Type three secretion system
Proinflammatory cytokine
03 medical and health sciences
medicine
Macrophage
Secretion
030304 developmental biology
Original Research
immune evasion
0303 health sciences
030306 microbiology
Pseudomonas aeruginosa
Chemistry
Biofilm
biochemical phenomena, metabolism, and nutrition
PcrV
Subjects
Details
- Language :
- English
- ISSN :
- 1664302X
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology
- Accession number :
- edsair.doi.dedup.....458a2f27551cb5de37a6df57a1ecb4d3