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Production of fermented cheese whey-based beverage using kefir grains as starter culture : evaluation of morphological and microbial variations
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2010
- Publisher :
- Elsevier, 2010.
-
Abstract
- Whey valorization concerns have led to recent interest on the production of whey beverage simulating kefir. In this study, the structure and microbiota of Brazilian kefir grains and beverages obtained from milk and whole/deproteinised whey was characterized using microscopy and molecular techniques. The aim was to evaluate its stability and possible shift of probiotic bacteria to the beverages. Fluorescence staining in combination with Confocal Laser Scanning Microscopy showed distribution of yeasts in macro-clusters among the grain’s matrix essentially composed of polysaccharides (kefiran) and bacteria. Denaturing gradient gel electrophoresis displayed communities included yeast affiliated to Kluyveromyces marxianus, Saccharomyces cerevisiae, Kazachatania unispora, bacteria affiliated to Lactobacillus kefiranofaciens subsp. Kefirgranum, Lactobacillus kefiranofaciens subsp. Kefiranofaciens and an uncultured bacterium also related to the genus Lactobacillus. A steady structure and dominant microbiota, including probiotic bacteria, was detected in the analyzed kefir beverages and grains. This robustness is determinant for future implementation of whey-based kefir beverages.<br />Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), CAPES-GRICES.
- Subjects :
- 0106 biological sciences
Environmental Engineering
Cultured Milk Products
ved/biology.organism_classification_rank.species
Bioengineering
CSLM
01 natural sciences
Beverages
03 medical and health sciences
fluids and secretions
Kefir
Species Specificity
Kluyveromyces marxianus
Cheese
010608 biotechnology
Food science
Cheese whey
Waste Management and Disposal
030304 developmental biology
2. Zero hunger
PCR-DGGE
0303 health sciences
Science & Technology
biology
Renewable Energy, Sustainability and the Environment
ved/biology
food and beverages
Kefiran
General Medicine
biology.organism_classification
Yeast
Bacteria, Aerobic
Milk
Fermentation
Temperature gradient gel electrophoresis
Bacteria
Lactobacillus kefiranofaciens
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....45fd7cbf82607ca80a335f0168d3c913