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Neuroregeneration of Induced Pluripotent Stem Cells in Polyacrylamide-Chitosan Inverted Colloidal Crystal Scaffolds with Poly(lactide-co-glycolide) Nanoparticles and Transactivator of Transcription von Hippel-Lindau Peptide
- Source :
- Tissue Engineering Part A. 23:263-274
- Publication Year :
- 2017
- Publisher :
- Mary Ann Liebert Inc, 2017.
-
Abstract
- Polyacrylamide (PAAM) and chitosan were fabricated by inverted colloidal crystal (ICC) method for scaffolds comprising regular pores. The hybrid PAAM-chitosan ICC scaffolds were grafted with poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) for a rougher pore surface and grafted with transactivator of transcription von Hippel-Lindau (TATVHL) peptide for a better differentiation of induced pluripotent stem (iPS) cells toward neural lineage. By scanning electron microscopy, we found that iPS cells cultured in PAAM-chitosan ICC scaffolds with PLGA NPs at 1.0 mg/mL and TATVHL peptide at 15 μg/mL elongated the axonal length to 15 μm. A combination of PLGA NPs and TATVHL peptide favored the adhesion of iPS cells, reduced the embryonic phenotype after cultivation, and guided the production of βIII tubulin-positive cells in PAAM-chitosan ICC scaffolds. In addition to the differentiation toward neurite-like cells, an increase in the content of TATVHL peptide in PAAM-chitosan ICC scaffolds inhibited the differentiation of iPS cells toward astrocytes. ICC scaffolds composed of PAAM, chitosan, PLGA NPs, and TATVHL peptide can be an efficacious matrix to differentiate iPS cells toward neurons and retard the glial formation for nerve regeneration.
- Subjects :
- 0301 basic medicine
Induced Pluripotent Stem Cells
Polyacrylamide
Acrylic Resins
Biomedical Engineering
Nanoparticle
Bioengineering
Peptide
macromolecular substances
02 engineering and technology
Biochemistry
Biomaterials
Chitosan
03 medical and health sciences
chemistry.chemical_compound
Transactivation
Transcription (biology)
Cell Adhesion
Animals
Humans
Induced pluripotent stem cell
Polyglactin 910
chemistry.chemical_classification
Tissue Engineering
Tissue Scaffolds
technology, industry, and agriculture
Cell Differentiation
equipment and supplies
021001 nanoscience & nanotechnology
Molecular biology
Neuroregeneration
carbohydrates (lipids)
030104 developmental biology
chemistry
Astrocytes
Microscopy, Electron, Scanning
Biophysics
Nanoparticles
0210 nano-technology
Subjects
Details
- ISSN :
- 1937335X and 19373341
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Tissue Engineering Part A
- Accession number :
- edsair.doi.dedup.....4611e3220a30ce9034f50b74d0c7e0e7