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Effect of Dexmedetomidine on Ischemia-Reperfusion Injury in Rat Kidney: A Histopathologic Study
- Source :
- Renal Failure. 31:70-74
- Publication Year :
- 2009
- Publisher :
- Informa UK Limited, 2009.
-
Abstract
- H, Ozturk/0000-0001-5608-5742 WOS: 000262472300012 PubMed: 19142813 Ischemia-reperfusion (I-R) injury remains the leading cause of acute renal failure. The purpose of this experimental study was to determine the role of dexmedetomidine on histologic alterations induced by renal I-R in rats. In the present study, thirty male Sprague-Dawley rats weighing 200-220 g were randomly assigned into three groups: the sham-control group (group 1, n = 10), the R/untreated group (group 2, n = 10), and the I-R/dexmedetomidine-treated group (group 3, n = 10). For group one, we performed a sham operation. The abdomen was dissected, the right kidney was harvested, and then the left renal pedicle exposed. Renal clamping was not applied. For group 2, rats underwent left renal ischemia for 60 minutes followed by reperfusion for 45 minutes. For group 3, the same surgical procedure as in group 2 was performed, and dexmedetomidine (100 g/kg, intraperitoneal) was administrated at the starting time of reperfusion. The rats were sacrificed after reperfusion, and the kidney tissue was harvested. The histopathological score in the kidney of the I-R/dexmedetomidine-treated group rats was significantly lower than that of I-R/untreated group rats. This score in I-R/untreated group rats was higher than the other two groups, which was statistically significant. In the I-R/untreated group rats, kidneys of untreated ischemia rats showed tubular cell swelling, cellular vacuolization, pyknotic nuclei, medullary congestion, and moderate to severe necrosis. Treatment with dexmedetomidine shows normal glomeruli and slight edema of the tubular cells. These findings provide the first evidence that dexmedetomidine can reduce the renal injury caused by I-R of the kidney, and may be useful in enhancing the tolerance of the kidney against renal injury.
- Subjects :
- Male
Nephrology
kidney
medicine.medical_specialty
renal ischemia-reperfusion injury
Urinary system
Kidney Glomerulus
Ischemia
Kidney
Critical Care and Intensive Care Medicine
Rats, Sprague-Dawley
Internal medicine
medicine
Animals
Dexmedetomidine
Renal ischemia
business.industry
dexmedetomidine
General Medicine
Acute Kidney Injury
medicine.disease
Rats
Kidney Tubules
medicine.anatomical_structure
Reperfusion Injury
Anesthesia
Renal Ischemia-reperfusion Injury
business
Adrenergic alpha-Agonists
Reperfusion injury
medicine.drug
Kidney disease
Subjects
Details
- ISSN :
- 15256049 and 0886022X
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Renal Failure
- Accession number :
- edsair.doi.dedup.....462d3bd03e5132a5961e413617650d15