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Paper-Based Strips for the Electrochemical Detection of Single and Double Stranded DNA
- Source :
- Analytical chemistry. 90(22)
- Publication Year :
- 2018
-
Abstract
- The detection of double stranded DNA (dsDNA) is often associated with the use of laboratory-bound approaches and/or with the prior generation of single stranded DNA (ssDNA), making these methods not suitable for in situ monitoring, i.e., point-of-care diagnostics. Screen-printed technology, coupled to the use of triplex forming oligonucleotides (TFO) as the recognizing probes, offers a great possibility toward the development of portable analytical tools. Moreover, the continuous demand for sustainable processes and waste lowering have highlighted the role of paper-based substrates for manufacturing easy-to-use, low-cost, and sustainable electrochemical devices. In this work, filter paper and copy paper have been utilized to produce E-DNA strips. Gold nanoparticles (AuNPs) have been exploited to immobilize the methylene blue (MB)-tagged TFO and to enhance the charge transfer kinetics at the electrode surface. Both paper-based substrates have been electrochemically characterized, and in addition, the effect of the amount of waxed layers has been evaluated. The paper-based E-DNA strips have been challenged toward the detection of three model targets, obtaining 3 and 7 nM as the detection limit, respectively, for single and double stranded sequences. The repeatability of the manufacturing (homemade) process has been evaluated with a relative standard deviation of approximately 10%. The effectiveness of the filter paper-based platform has been also evaluated in undiluted serum obtaining a similar value of the detection limit (compared to the measurements carried out in buffer solution). In addition, a synthetic PCR amplified dsDNA sequence, related to HIV, has been detected in serum samples.
- Subjects :
- Paper
Electrode
Reproducibility of Result
DNA, Single-Stranded
Metal Nanoparticles
Nanotechnology
STRIPS
010402 general chemistry
Electrochemistry
01 natural sciences
Analytical Chemistry
law.invention
chemistry.chemical_compound
Metal Nanoparticle
law
Settore CHIM/01 - Chimica Analitica
Electrodes
Kinetic
Filter paper
Electrochemical Technique
Oligonucleotide
010401 analytical chemistry
Solid Phase Extraction
HIV
Reproducibility of Results
DNA
Electrochemical Techniques
0104 chemical sciences
Kinetics
chemistry
Colloidal gold
Costs and Cost Analysi
Costs and Cost Analysis
Gold
Double stranded
Subjects
Details
- ISSN :
- 15206882
- Volume :
- 90
- Issue :
- 22
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....4668984328897b16a1ad1f6cb444ef5d