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Detection of fluid secretion of three-dimensional reconstructed eccrine sweat glands by magnetic resonance imaging
- Source :
- Experimental Dermatology. 28:53-58
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- We previously showed three-dimensional (3D) reconstructed eccrine sweat glands have similar structures as native eccrine sweat glands, but whether the 3D reconstructed sweat glands appropriately secrete fluid is still unknown. In this study, Matrigel-embedded human eccrine sweat gland cells or Matrigel alone were implanted into the groin subcutis of the nude mice. Ten weeks post-implantation, images of the subcutaneously formed plugs, as well as footpads of rats, pre- and post-pilocarpine/normal saline (NS) injection were acquired using a fat-suppressed proton density-weighted magnetic resonance imaging (MRI) sequence at 7.0 T, and the regions of interest (ROIs) in plugs and rat footpads were analysed and graphed. A significant increase in the ROI mean proton intensity occurred in both 3D reconstructed and native eccrine sweat glands after pilocarpine injection. The mean proton intensity had no noticeable changes in ROIs of Matrigel plugs between pre- and post-pilocarpine injection, and in ROIs of rat footpads between pre- and post-NS injection. In conclusion, the 3D reconstructed sweat glands possess fluid secretion, which is detectable by fat-suppressed proton density-weighted MRI.
- Subjects :
- 0301 basic medicine
Adolescent
medicine.medical_treatment
Eccrine sweat
Dermatology
Eccrine Glands
Muscarinic Agonists
Biochemistry
Rats, Sprague-Dawley
SWEAT
Mice
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
Secretion
Eccrine sweat gland
Child
Molecular Biology
Saline
Cells, Cultured
Mice, Inbred BALB C
Matrigel
medicine.diagnostic_test
Chemistry
Pilocarpine
Infant
Magnetic resonance imaging
Anatomy
Magnetic Resonance Imaging
Body Fluids
Rats
Drug Combinations
030104 developmental biology
medicine.anatomical_structure
Child, Preschool
Proteoglycans
Collagen
Laminin
Protons
medicine.drug
Subjects
Details
- ISSN :
- 09066705
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Experimental Dermatology
- Accession number :
- edsair.doi.dedup.....466bd774e1f32cc26a69e6ac577904f2