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Growth-promoting action and growth factor release by different platelet derivatives
- Source :
- Platelets (Sheff.) (2014)., info:cnr-pdr/source/autori:Passaretti F, Tia M, D'Esposito V, Pascale MD, Corso MD, Sepulveres R, Liguoro D, Valentino R, Beguinot F, Formisano P, Sammartino G./titolo:Growth-promoting action and growth factor release by different platelet derivatives./doi:/rivista:Platelets (Sheff.)/anno:2014/pagina_da:/pagina_a:/intervallo_pagine:/volume
- Publication Year :
- 2013
-
Abstract
- Platelet derivatives are commonly used in wound healing and tissue regeneration. Different procedures of platelet preparation may differentially affect growth factor release and cell growth. Preparation of platelet-rich fibrin (PRF) is accompanied by release of growth factors, including platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and transforming growth factor ?1 (TGF?1), and several cytokines. When compared with the standard procedure for platelet-rich plasma (PRP), PRF released 2-fold less PDGF, but >15-fold and >2-fold VEGF and TGF?1, respectively. Also, the release of several cytokines (IL-4, IL-6, IL-8, IL-10, IFN?, MIP-1?, MIP-1? and TNF?) was significantly increased in PRF-conditioned medium (CM), compared to PRP-CM. Incubation of both human skin fibroblasts and human umbilical vein endothelial cells (HUVECs) with PRF-derived membrane (mPRF) or with PRF-CM enhanced cell proliferation by >2-fold (p < 0.05). Interestingly, PRP elicited fibroblast growth at a higher extent compared to PRF. At variance, PRF effect on HUVEC growth was significantly greater than that of PRP, consistent with a higher concentration of VEGF in the PRF-CM. Thus, the procedure of PRP preparation leads to a larger release of PDGF, as a possible result of platelet degranulation, while PRF enhances the release of proangiogenic factors.
- Subjects :
- Adult
Blood Platelets
Male
medicine.medical_specialty
medicine.medical_treatment
Biology
chemistry.chemical_compound
Young Adult
Platelet degranulation
Internal medicine
medicine
Human Umbilical Vein Endothelial Cells
Humans
Platelet
Cell Proliferation
Platelet-Rich Plasma
Growth factor
Hematology
General Medicine
Fibroblasts
digestive system diseases
Vascular endothelial growth factor
Endocrinology
chemistry
Platelet-rich plasma
biology.protein
Cytokines
Intercellular Signaling Peptides and Proteins
Female
Wound healing
Platelet-derived growth factor receptor
Transforming growth factor
Subjects
Details
- ISSN :
- 13691635
- Volume :
- 25
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Platelets
- Accession number :
- edsair.doi.dedup.....46818ebf24d1780d3c9ab9a754c19021