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Metabolic engineering to improve 1,5-diaminopentane production from cellobiose using β-glucosidase-secreting Corynebacterium glutamicum
- Source :
- Biotechnology and Bioengineering. 116(10):2640-2651
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Microbial production of 1,5-diaminopentane (DAP) from renewable feedstock is a promising and sustainable approach for the production of polyamides. In this study, we constructed a beta-glucosidase (BGL)-secreting Corynebacterium glutamicum and successfully used this strain to produce DAP from cellobiose and glucose. First, C. glutamicum was metabolically engineered to produce l-lysine (a direct precursor of DAP), followed by the coexpression of l-lysine decarboxylase and BGL derived from Escherichia coli and Thermobifida fusca YX (Tfu0937), respectively. This new engineered C. glutamicum strain produced 27 g/L of DAP from cellobiose in CGXII minimal medium using fed-batch cultivation. The yield of DAP was 0.43 g/g glucose (1 g of cellobiose corresponds to 1.1 g of glucose), which is the highest yield reported to date. These results demonstrate the feasibility of DAP production from cellobiose or cellooligosaccharides using an engineered C. glutamicum strain.
- Subjects :
- Thermobifida fusca
Strain (chemistry)
beta-Glucosidase
Bioengineering
Cellobiose
Diamines
medicine.disease_cause
Applied Microbiology and Biotechnology
Corynebacterium glutamicum
1,5-diaminopentane
Metabolic engineering
chemistry.chemical_compound
Bacterial Proteins
Biochemistry
chemistry
cellobiose
Yield (chemistry)
β-glucosidase
medicine
bacteria
metabolic engineering
Escherichia coli
β glucosidase
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 00063592
- Volume :
- 116
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Biotechnology and Bioengineering
- Accession number :
- edsair.doi.dedup.....46b0ed53968e576182989b207066ebd0