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Cervical microbiome is altered in cervical intraepithelial neoplasia after loop electrosurgical excision procedure in china
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Although human papillomavirus (HPV) infection is a major cause leading to the development of cervical intraepithelial neoplasia (CIN), the relationship between genital microbiome and HPV persistence/clearance is not well established. Loop electrosurgical excision procedure (LEEP) is one of standard treatments of CIN 2/3 globally, yet little is known about how the LEEP influence genital microbiota. We conducted a prospective study of 26 patients with CIN2/3 who underwent analysis of cervical microbiome before and after 3 months of LEEP treatment. Cervical swabs were collected, and microbiomes were analyzed by 16S ribosomal RNA gene sequencing. A decrease of cervical microbial diversity was observed after 3 months of LEEP treatment. Notably, a significant shift from community type of a Prevotella-containing and lack of a consistent dominant species to lactobacillus iners dominated microbiome correlated with LEEP. Particularly, Leptotrichia and clostridium were further decreased after LEEP treatment (P = 0.049 and P = 0.002, respectively). Our results suggest that the cervical microbiome is altered after LEEP treatment in patients with CIN2/3. Further studies with larger sample sizes are needed to validate these findings.
- Subjects :
- Adult
0301 basic medicine
medicine.medical_specialty
Electrosurgery
medicine.medical_treatment
lcsh:Medicine
Uterine Cervical Neoplasms
Cervical intraepithelial neoplasia
Gastroenterology
Article
03 medical and health sciences
0302 clinical medicine
RNA, Ribosomal, 16S
Internal medicine
medicine
Lactobacillus iners
Humans
Sex organ
Prospective Studies
Microbiome
lcsh:Science
Prospective cohort study
Leptotrichia
Aged
Multidisciplinary
Bacteria
biology
business.industry
Microbiota
lcsh:R
Middle Aged
Uterine Cervical Dysplasia
biology.organism_classification
medicine.disease
RNA, Bacterial
030104 developmental biology
Loop electrosurgical excision procedure
030220 oncology & carcinogenesis
lcsh:Q
Female
business
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....46b5343f85e1f20e96c38b2ef1407147