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Growth suppression of human laryngeal squamous cell carcinoma by adenovirus-mediated tissue factor pathway inhibitor gene 2
- Source :
- The Laryngoscope. 116(4)
- Publication Year :
- 2006
-
Abstract
- Objective: The purpose of this study was to explore the inhibiting role of adenoviral-mediated (Ad)-tissue factor pathway inhibitor (TFPI)-2 gene in the growth of laryngeal squamous cell carcinoma (LSCC). Study Design: The nude mice bearing LSCC were prepared by intracutaneous injection of Hep-2 cell. Gene therapy was performed by injecting adenoviruses carrying TFPI-2 gene around tumors in the animal model. Methods: Eighteen nude mice bearing Hep-2 cell tumor were randomly separated into the treated group and control group. The former were injected with recombinant adenovirus Ad-TFPI-2 around the tumor, and the later were injected with equivalent Ad-LacZ. After treatment, differences of tumor weight, volume, and ultrastructure of tumor cells between these two groups were observed by measuring and using transmission electron microscope. Apoptosis in Hep-2 xenotransplants was detected using the terminal deoxy-transferase-mediated dUTP nick end labeling. In addition, the expressions of TFPI-2 protein and proliferating cell nuclear antigen (PCNA) in tumor tissues were detected using Western blot and immunohistochemistry, respectively. Results: The average weight and volume of tumor in the treated group were significantly lower than that in the control group (P < .01). The PCNA index was obviously lower in the tumors treated group than that in the control group (P < .01). In addition, cell apoptosis was observed in xenotransplants of the treated group but not in the control group. Conclusion: Peritumor injection of Ad-TFPI-2 can inhibit growth of LSCC in nude mice model, and TFPI-2 might be a desirable gene for gene therapy in LSCC.
- Subjects :
- Pathology
medicine.medical_specialty
Serine Proteinase Inhibitors
Genetic enhancement
Cell
Blotting, Western
Genetic Vectors
Apoptosis
Factor VIIa
medicine.disease_cause
Adenoviridae
Mice
Tissue factor pathway inhibitor
Western blot
Microscopy, Electron, Transmission
Cell Line, Tumor
Proliferating Cell Nuclear Antigen
medicine
Animals
Humans
Laryngeal Neoplasms
Glycoproteins
Mice, Inbred BALB C
biology
medicine.diagnostic_test
business.industry
Genetic Therapy
Immunohistochemistry
Proliferating cell nuclear antigen
medicine.anatomical_structure
Otorhinolaryngology
biology.protein
Cancer research
Carcinoma, Squamous Cell
business
Subjects
Details
- ISSN :
- 0023852X
- Volume :
- 116
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- The Laryngoscope
- Accession number :
- edsair.doi.dedup.....46b993b9fad4ba6363532fdb0a0ba603