Additional file 2 of Endoplasmic reticulum stress promotes the release of exosomal PD-L1 from head and neck cancer cells and facilitates M2 macrophage polarization

Additional file 1. Fig. S1. IFN-�� did not affect PD-L1 expression in the treatment macrophages. (A) The THP-1 macrophages were treated with Exo-ER, IFN-��, or PBS (Blank), followed by the detection of the PD-L1 mRNA (A) and protein levels (B and C) (n = 2-3) (*P < 0.05 and **P < 0.01; NS, no significance). Fig. S2. Knockdown of PD-L1 in HN4 cells and HN4-derived exosomes. HN4 cells were transfected with siPD-L1 or siNC, and exosomes were collected. (A-C) PD-L1 mRNA and protein level were detected in treatment HN4 cells by qRT-PCR (A) and Western blot (B and C). (D-E) Exosomal PD-L1 protein level was detected in treatment HN4 cells (n = 2-3) (*P < 0.05 and **P < 0.01). Fig. S3. Overexpression of PD-L1 promoted M2 polarization in macrophages. qRT-PCR analysis of CD206 mRNA in THP-1 macrophages transfected with plasmid encoding control (OE-NC) or PD-L1 (OE-PD-L1) (n = 3) (**P < 0.01). Table S1. RNA sequence used in this paper. Table S2. Clinicopathologic features of OSCC patients.