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CgSOCS6 negatively regulates the expression of CgIL17s and CgDefh1 in the pacific oyster Crassostrea gigas
- Source :
- Fishshellfish immunology. 93
- Publication Year :
- 2019
-
Abstract
- As a family of negatively feedback regulating factors, the suppressor of cytokine signaling (SOCS) can depress cytokine signal transduction, and eventually modulate growth, development, differentiation, and immune response. In the present study, a SOCS homologue (designated as CgSOCS6) was identified from oyster Crassostrea gigas. The open reading frame of CgSOCS6 cDNA was of 1167 bp encoding a peptide of 388 amino acid residues with a central Src homology 2 (SH2) domain, a conserved C-terminal SOCS box, and a nucleus localization sequence (NLS) in its N-terminus. The deduced amino acid sequence of CgSOCS6 shared 37.9-45.5% similarity with other SOCS6/7 family members. In the unrooted phylogenetic tree, CgSOCS6 was clustered with EsSOCS6 from Chinese mitten crab Eriocheir sinensis and assigned into the SOCS6/7 group. The mRNA transcripts of CgSOCS6 were constitutively distributed in all the tested tissues, with the highest level in hemocytes. After lipopolysaccharide (LPS) stimulation, the mRNA expression of CgSOCS6 in hemocytes was significantly up-regulated to the highest level at 6 h (8.48-fold compared to the control group, p 0.01), and then kept at a relatively higher level from 12 h to 72 h. CgSOCS6 protein could be translocated into the hemocyte nucleus after LPS stimulation. The mRNA expressions of interleukin 17-4 (CgIL17-4), CgIL17-5, and defensin (CgDefh1) in the hemocytes of CgSOCS6-knockdown oysters increased significantly (2.55-fold, 2.68-fold, 4.68-fold of that in EGFP-RNAi oysters, p 0.05, p 0.05, p 0.001, respectively) after LPS stimulation. These findings suggested that CgSOCS6 was involved in the oyster immune response by regulating the expressions of CgIL17-4, CgIL17-5, and CgDefh1.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Suppressor of Cytokine Signaling Proteins
Aquatic Science
Biology
Homology (biology)
Defensins
03 medical and health sciences
Complementary DNA
Environmental Chemistry
Animals
SOCS6
Amino Acid Sequence
Crassostrea
Peptide sequence
Phylogeny
Messenger RNA
Nucleus localization
Gene Expression Profiling
Interleukin-17
04 agricultural and veterinary sciences
General Medicine
biology.organism_classification
Immunity, Innate
Cell biology
Open reading frame
030104 developmental biology
Gene Expression Regulation
040102 fisheries
0401 agriculture, forestry, and fisheries
Sequence Alignment
Subjects
Details
- ISSN :
- 10959947
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Fishshellfish immunology
- Accession number :
- edsair.doi.dedup.....46eadb405c0a390c91d364b91993d122