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Effects of epidermal growth factor and erythropoietin on Müller glial activation and phenotypic plasticity in the adult mammalian retina
- Source :
- Journal of Neuroscience Research. 89:1018-1030
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- Retinal Müller glia have received considerable attention with regard to their potential to function as quiescent retinal precursors. Various activation strategies induce characteristic features of retinal progenitor cells in Müller glia; however, these are often accompanied by hallmark features of reactive gliosis. We investigated the effects of an intravitreal injection of epidermal growth factor (EGF), a known mitogen, and erythropoietin (EPO) on activation and expression of developmental phenotypes within the adult retina. Using thymidine-analogue labeling as well as immunocytochemical and confocal analyses, we assayed the responses of retinal cells exposed to intravitreal administration of either EGF or EPO. We report that adult Müller glia incorporate bromodeoxyuridine (BrdU) and undergo a process of nuclear translocation to ectopic retinal layers following exposure to EGF. These cells survive within the retina for at least 23 days and express the developmental markers Pax6 and Chx10 as well as nestin and glial fibrillary acidic protein. Furthermore, we demonstrate that cotreatment with EGF and EPO suppresses aspects of EGF-induced glial reactivity, alters the retinal distribution of BrdU-positive nuclei, and serves to regulate the expression of developmental phenotypes seen in these cells. These data further our understanding of Müller cell responsiveness to intravitral, combinatorial growth factor treatments.
- Subjects :
- Cell Survival
medicine.medical_treatment
Biology
Retina
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Neural Stem Cells
Epidermal growth factor
medicine
Animals
Rats, Wistar
Erythropoietin
Neuronal Plasticity
Epidermal Growth Factor
Glial fibrillary acidic protein
Growth factor
Age Factors
Cell Differentiation
Retinal
Nestin
Rats
Cell biology
Phenotype
medicine.anatomical_structure
chemistry
biology.protein
Female
sense organs
PAX6
Neuroglia
Muller glia
Subjects
Details
- ISSN :
- 03604012
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Research
- Accession number :
- edsair.doi.dedup.....46fc20092e7376a634aa7f58938237c2
- Full Text :
- https://doi.org/10.1002/jnr.22629