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Initiation of RNA replication of cloned Taiwan-3 isolate of hepatitis delta virus genotype II in cultured cells
- Source :
- Biochemical and biophysical research communications. 306(4)
- Publication Year :
- 2003
-
Abstract
- Hepatitis delta virus (HDV) genotype II is the predominant genotype in Taiwan and is associated with less progressive disease than genotype I. Although the Taiwan-3 (T3) clone was the first genotype II HDV isolated in Taiwan, its replication in cultured cells has not previously been established. Here, we demonstrate that cloned T3 HDV is capable of replicating in cultured cells. Furthermore, we show that: (1) the replication level of T3 clones is 100-fold lower than that of a genotype I HDV prototype of Italian origin; (2) both forms of the genotype II T3 delta antigen are expressed; and (3) T3 HDV undergoes RNA editing during replication, with 4.8% of the T3 genomes showing evidence of editing. The low level of RNA replication may be related to the milder clinical outcomes of genotype II HDV infections.
- Subjects :
- Genotype
viruses
Blotting, Western
Molecular Sequence Data
Biophysics
Clone (cell biology)
Biology
Transfection
Virus Replication
Biochemistry
Genome
Virus
Animals
Cloning, Molecular
Molecular Biology
Genetics
Delta Antigen
Deoxyribonucleases
Base Sequence
Models, Genetic
HEPATITIS DELTA
virus diseases
Cell Biology
biochemical phenomena, metabolism, and nutrition
Virology
Hepatitis D
RNA editing
COS Cells
RNA
RNA, Viral
Hepatitis Delta Virus
Plasmids
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 306
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....46fd92862f838be49f93c75fd531dc57