Back to Search
Start Over
Nitric oxide in the sensory function of the carotid body
- Source :
- Brain Research. 625:16-22
- Publication Year :
- 1993
- Publisher :
- Elsevier BV, 1993.
-
Abstract
- Recent studies suggest that nitric oxide (NO) may act as a chemical messenger in the nervous system. Since neurotransmitters are considered necessary for the sensory function of the carotid body, and molecular O 2 is a co-factor for NO synthesis, we examined whether (a) chemoreceptor tissue also synthesizes NO and if so, (b) does endogenous NO affect chemosensory activity. Experiments were performed on carotid bodies obtained from anesthetized cats ( n = 20 ). Distribution of nitric oxide synthase (NOS), an enzyme that catalyzes the formation of NO was examined using NADPH-diaphorase histochemistry. Many nerve plexuses innervating the chemoreceptor tissue were positive for NADPH-diaphorase, indicating that the nerve fibers are the primary source of NO production in the carotid body. Radiometric analysis of NOS activity of the chemoreceptor tissue averaged 1.94 pmol [ 3 H]citrulline/min/mg protein. NOS activity was significantly less in low pO 2 reaction medium than in room air controls. Chemosensory activity in vitro increased in a dose-dependent manner in response to l -ω-nitro arginine ( l -NNA), an inhibitor of NOS activity. The effects of NOS inhibitor were enantiomer selective as evidence by reversal of the responses by l - but not d -arginine. These observations imply that endogenous NO is inhibitory to carotid body sensory activity. cGMP levels of l -NNA-treated carotid bodies were significantly less than untreated controls, suggesting that the actions of NO are coupled to the cGMP second messenger system, as elsewhere in the nervous system. Based on the findings that (a) low pO 2 decreased NOS activity and, (b) endogenous NO is inhibitory to the sensory discharge, it is suggested that the mechanism(s) of sensory excitation by hypoxia in the carotid body may involve ‘disinhibition’ inhibitory to the sensory discharge, it is suggested that the mechanism(s) of sensory excitation by hypoxia in the carotid body may involve ‘disinhibition’ resulting from NO synthesis.
- Subjects :
- Male
Nervous system
medicine.medical_specialty
Chemoreceptor
Arginine
Partial Pressure
Nitric Oxide
Nitroarginine
Nitric oxide
chemistry.chemical_compound
Internal medicine
medicine
Citrulline
Animals
Tissue Distribution
Cyclic GMP
Molecular Biology
Carotid Body
biology
Chemistry
General Neuroscience
NADPH Dehydrogenase
Stereoisomerism
Chemoreceptor Cells
Oxygen
Nitric oxide synthase
medicine.anatomical_structure
Endocrinology
Second messenger system
Cats
biology.protein
Female
Carotid body
Amino Acid Oxidoreductases
Neurology (clinical)
Nitric Oxide Synthase
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 625
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....46fedea746ca27cfaa466f85c2f16b5c
- Full Text :
- https://doi.org/10.1016/0006-8993(93)90132-7