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OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human
- Source :
- Nature Communications, Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021)
- Publication Year :
- 2021
-
Abstract
- Understanding the molecular underpinnings of pluripotency is a prerequisite for optimal maintenance and application of embryonic stem cells (ESCs). While the protein-protein interactions of core pluripotency factors have been identified in mouse ESCs, their interactome in human ESCs (hESCs) has not to date been explored. Here we mapped the OCT4 interactomes in naïve and primed hESCs, revealing extensive connections to mammalian ATP-dependent nucleosome remodeling complexes. In naïve hESCs, OCT4 is associated with both BRG1 and BRM, the two paralog ATPases of the BAF complex. Genome-wide location analyses and genetic studies reveal that these two enzymes cooperate in a functionally redundant manner in the transcriptional regulation of blastocyst-specific genes. In contrast, in primed hESCs, OCT4 cooperates with BRG1 and SOX2 to promote chromatin accessibility at ectodermal genes. This work reveals how a common transcription factor utilizes differential BAF complexes to control distinct transcriptional programs in naïve and primed hESCs.<br />Although the interactors of pluripotency factors have been identified in mouse embryonic stem cells (ESCs), their interactors in human ESCs remain unexplored. Here the authors map OCT4 protein interactions in naïve and primed human ESCs to find specific interactions with BAF subunits that promote an open chromatin architecture at blastocyst-associated genes and ectodermal genes, respectively.
- Subjects :
- endocrine system
Embryonic stem cells
Science
General Physics and Astronomy
Chromatin remodelling
Biology
Interactome
General Biochemistry, Genetics and Molecular Biology
Article
Adenosine Triphosphate
SOX2
Transcriptional regulation
Nucleosome
Humans
Gene
Transcription factor
reproductive and urinary physiology
Multidisciplinary
urogenital system
SOXB1 Transcription Factors
DNA Helicases
Nuclear Proteins
General Chemistry
Chromatin Assembly and Disassembly
Embryonic stem cell
Chromatin
Cell biology
Nucleosomes
Gene Expression Regulation
embryonic structures
biological phenomena, cell phenomena, and immunity
Octamer Transcription Factor-3
Protein Binding
Transcription Factors
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature communications
- Accession number :
- edsair.doi.dedup.....4704ae735085e5f0f05f17096ca08183