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Intramolecular subunit interactions between insulin and insulin-like growth factor 1 .alpha..beta. half-receptors induced by ligand and manganese/magnesium-ATP binding
- Source :
- Biochemistry. 31:11801-11805
- Publication Year :
- 1992
- Publisher :
- American Chemical Society (ACS), 1992.
-
Abstract
- We have previously demonstrated that isolated insulin and IGF-1 alpha beta half-receptors can be reconstituted into a functional alpha 2 beta 2 hybrid receptor complex [Treadway et al. (1989) J. Biol. Chem. 264, 21450-21453]. In the present study, we have examined this assembly process by determining the effect of ligand occupancy and Mn/MgATP binding on the dimerization of mutant and wild-type insulin and IGF-1 alpha beta half-receptors. IGF-1 or Mn/MgAMPPCP binding to wild-type IGF-1 alpha beta half-receptors resulted in the specific assembly of the alpha beta half-receptors into an alpha 2 beta 2 heterotetrameric IGF-1 holoreceptor complex. Similarly, insulin binding to the kinase-deficient mutant (A/K1018) insulin alpha beta half-receptor also resulted in the specific assembly into an alpha 2 beta 2 holoreceptor complex. In contrast, Mn/MgAMPPCP treatment of A/K1018 mutant insulin alpha beta half-receptors did not induce heterotetramer assembly, consistent with the inability of this mutant receptor to bind ATP. The ability of the insulin alpha beta receptors to assemble with the IGF-1 alpha beta half-receptors was used to examine the intermolecular subunit interactions responsible for dimerization. In the presence of Mn/MgAMPPCP, the wild-type insulin and wild-type IGF-1 alpha beta half-receptors were observed to assemble into an insulin/IGF-1 alpha 2 beta 2 hybrid receptor complex. Similarly, a combination of insulin and IGF-1 induced hybrid receptor formation between wild-type IGF-1 and A/K1018 mutant insulin alpha beta half-receptors.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Manganese
Receptor complex
Macromolecular Substances
Chemistry
Insulin
medicine.medical_treatment
Alpha (ethology)
Ligand (biochemistry)
Biochemistry
Receptor, Insulin
Receptor, IGF Type 1
Insulin-like growth factor
Adenosine Triphosphate
Cell surface receptor
Chromatography, Gel
medicine
Biophysics
Humans
Magnesium
Insulin-Like Growth Factor I
Protein Multimerization
Receptor
Beta (finance)
Subjects
Details
- ISSN :
- 15204995 and 00062960
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Biochemistry
- Accession number :
- edsair.doi.dedup.....47253499583b4c5b34e0c9669d37e4d8
- Full Text :
- https://doi.org/10.1021/bi00162a018