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l-Carnitine protects against apoptosis of murine MC3T3-E1 osteoblastic cells
- Source :
- Amino Acids. 35:419-423
- Publication Year :
- 2007
- Publisher :
- Springer Science and Business Media LLC, 2007.
-
Abstract
- L-carnitine (LC), an amino acid with a major role in cellular energy metabolism, has positive effects on bone metabolism. However, the effect of LC on apoptosis of osteoblast in vitro has not been reported. The aim of this study was to investigate the action of LC on apoptosis of mouse osteoblastic cell line MC3T3-E1. Cell apoptosis was measured by sandwich-enzyme-immunoassay. Release of cytochrome c from mitochondria into cytosol and Bcl-2, Bax protein levels were determined by Western blot analysis. The enzyme substrate was used to assess the activation of caspase-3 and caspase-9. LC inhibited MC3T3-E1 cell apoptosis induced by serum deprivation. Our study also shows that LC decreased cytochrome c release and caspase-3 and caspase-9 activation in serum-deprived MC3T3-E1 cells. Furthermore, LC protected against MC3T3-E1 cell apoptosis induced by the glucocorticoid (GC) dexamethasone (Dex).
- Subjects :
- Blotting, Western
Clinical Biochemistry
Apoptosis
Mitochondrion
Biology
Biochemistry
Dexamethasone
Cell Line
Mice
Structure-Activity Relationship
Western blot
Carnitine
medicine
Animals
bcl-2-Associated X Protein
chemistry.chemical_classification
Osteoblasts
Dose-Response Relationship, Drug
medicine.diagnostic_test
Cytochrome c
Organic Chemistry
Cytochromes c
Osteoblast
Metabolism
Molecular biology
Culture Media
Cytosol
medicine.anatomical_structure
Enzyme
Proto-Oncogene Proteins c-bcl-2
chemistry
Caspases
biology.protein
Subjects
Details
- ISSN :
- 14382199 and 09394451
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Amino Acids
- Accession number :
- edsair.doi.dedup.....47694a4250749a9bb0367e0c8a705e8c