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L-carnitine suppresses cisplatin-induced renal injury in rats: impact on cytoskeleton proteins expression
- Source :
- Toxicol Res (Camb)
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- We designed this work to examine the curative role of L-carnitine (LCAR) in a rat model of cisplatin (CDDP)-induced kidney injury. We induced kidney injury in rats by a single intraperitoneal injection of 5 mg/kg of CDDP. Fifteen days post injection, rats were orally supplemented with 354 mg/kg of LCAR for another 15 days. Kidney tissues were subjected to histo-biochemical analysis along with mRNA gene expression quantification for cytoskeleton proteins encoding genes (vimentin, nestin, and connexin 43) by real-time reverse transcription polymerase chain reaction. LCAR reversed CDDP-induced renal structural and functional impairments. LCAR significantly declined serum urea and creatinine concentrations, restored oxidant/antioxidant balance, reversed inflammation, and antagonized caspase 3-mediated apoptotic cell death in renal tissues. Moreover, LCAR effectively down-regulated cytoskeleton proteins mRNA levels, reflecting amelioration of CDDP-provoked podocyte injury. We concluded that LCAR has a favorable therapeutic utility against CDDP-induced kidney injury.
- Subjects :
- Paper
Cisplatin
0303 health sciences
Creatinine
Kidney
biology
Health, Toxicology and Mutagenesis
medicine.medical_treatment
Intraperitoneal injection
Vimentin
Pharmacology
Toxicology
Reverse transcription polymerase chain reaction
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine.anatomical_structure
chemistry
030220 oncology & carcinogenesis
medicine
biology.protein
Carnitine
Caspase
030304 developmental biology
medicine.drug
Subjects
Details
- ISSN :
- 20454538
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Toxicology Research
- Accession number :
- edsair.doi.dedup.....478de45bb5fd738520f36af3f69a5d44