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PbCrO4 mediates cellular responses via reactive oxygen species
- Source :
- Molecular and cellular biochemistry. 255(1-2)
- Publication Year :
- 2004
-
Abstract
- Exposure to certain particulate hexavalent chromium [Cr(VI)] compounds, such as lead chromate (PbCrO4), has been associated with lung cancer and respiratory tract toxicity. Previous studies indicate that the solubility of Cr(VI)-compounds is an important factor in Cr(VI)-induced carcinogenesis. The present study investigates reactive oxygen species (ROS) generation by PbCrO4 particles and cellular responses using RAW 264.7 cells. A mixture containing PbCrO4 and RAW 264.7 cells generated hydroxyl radical ((.)OH), using cellularly generated H2O2 as a precursor, as measured by electron spin resonance (ESR) spin trapping in combination with H2O2 and (.)OH scavengers, catalase and sodium formate. The effect of ascorbic acid on (.)OH radicals was also measured using ESR. Confocal microscopy showed that particles could become either bound to the cell surface or engulfed over a 120 min time period. H2O2 generation and O2 consumption were also increased after treatment of the cells with PbCrO4. Both NF-kappaB and AP-1 were activated after exposure to PbCrO4 particles as measured by the NF-kappaB or AP-1 luciferase reporter plasmid assay. Our investigation thus demonstrated that the RAW 264.7 cells phagocytized the PbCrO4 particles leading to accumulation of the particles within vacuoles in the cytoplasm. These particles could induce chronic production of ROS and activation of NF-kappaB and AP-1. Such induction of transcription pathways may be involved in the inflammatory and carcinogenic responses induced by Cr(VI)-containing particles.
- Subjects :
- Radical
Clinical Biochemistry
Inorganic chemistry
Cell Line
chemistry.chemical_compound
Mice
Chromates
Animals
Hexavalent chromium
Luciferases
Molecular Biology
chemistry.chemical_classification
Reactive oxygen species
Spin trapping
biology
Sodium formate
Macrophages
Electron Spin Resonance Spectroscopy
NF-kappa B
Cell Biology
General Medicine
Hydrogen Peroxide
Ascorbic acid
Recombinant Proteins
Artificial Gene Fusion
Oxygen
Transcription Factor AP-1
chemistry
Lead
Catalase
biology.protein
Hydroxyl radical
Reactive Oxygen Species
Nuclear chemistry
Signal Transduction
Subjects
Details
- ISSN :
- 03008177
- Volume :
- 255
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular biochemistry
- Accession number :
- edsair.doi.dedup.....4797f7f24540ab20cacceef02de9f78a