Additional file 3 of Safety, recommended dose, efficacy and immune correlates for nintedanib in combination with pembrolizumab in patients with advanced cancers

Additional file 3: Supplementary Fig. 3. Illustration of multiplex chromogenic staining dedicated to myeloid cells. A Representative image displays a 500umx669um image after multispectral imaging and spectral unmixing (merged image). B All markers. C CD68 (pseudocoloured white). D DAPI nuclear marker (pseudocoloured blue). E CD163 (pseudocoloured green). F DC-LAMP (pseudocoloured magenta). G IDO1 (pseudocoloured yellow). H CD163 measurement was undertaken using Inform v2.2 software segmentation by pixel-based threshold. I CD163+ density in biopsies of patients with DCB or without DCB at baseline and C2D1 were not significantly different (Wilcoxon rank-sum test).