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Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis , a zoophytophagous predatory bug that is both a biological control agent and a pest
- Source :
- Insect Molecular Biology, 30, 188-209. Wiley, Insect Molecular Biology, Insect Molecular Biology 40 (2021) 2, Insect Molecular Biology, 40(2), 188-209
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Nesidiocoris tenuis (Reuter) is an efficient predatory biological control agent used throughout the Mediterranean Basin in tomato crops but regarded as a pest in northern European countries. From the family Miridae, it is an economically important insect yet very little is known in terms of genetic information and no genomic or transcriptomic studies have been published. Here, we use a linked‐read sequencing strategy on a single female N. tenuis. From this, we assembled the 355 Mbp genome and delivered an ab initio, homology‐based and evidence‐based annotation. Along the way, the bacterial “contamination” was removed from the assembly. In addition, bacterial lateral gene transfer (LGT) candidates were detected in the N. tenuis genome. The complete gene set is composed of 24 688 genes; the associated proteins were compared to other hemipterans (Cimex lectularis, Halyomorpha halys and Acyrthosiphon pisum). We visualized the genome using various cytogenetic techniques, such as karyotyping, CGH and GISH, indicating a karyotype of 2n = 32. Additional analyses include the localization of 18S rDNA and unique satellite probes as well as pooled sequencing to assess nucleotide diversity and neutrality of the commercial population. This is one of the first mirid genomes to be released and the first of a mirid biological control agent.<br />The annotated genome of predatory mirid Nesidiocoris tenuis presented here was generated from a single female insect using a linked‐read sequencing and assembly strategy.Potential contaminants and/or endosymbionts were identified during assembly decontamination along with putative lateral gene transfer candidates.Additional cytogenetic analysis includes karyotyping and investigations into the ancestral insect telomeric motif, sex chromosomes, rDNA and a very abundant DNA repeat scattered throughout the genome.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Gene Transfer, Horizontal
Genome, Insect
Population
Biology
Laboratorium voor Erfelijkheidsleer
010603 evolutionary biology
01 natural sciences
Genome
DNA sequencing
Nucleotide diversity
Population genomics
Heteroptera
Hemiptera
03 medical and health sciences
linked‐read
Genetics
Animals
biocontrol
Laboratory of Entomology
Symbiosis
education
Molecular Biology
Gene
030304 developmental biology
2. Zero hunger
linked-read
0303 health sciences
education.field_of_study
Bacteria
Original Articles
PE&RC
Laboratorium voor Entomologie
biology.organism_classification
Miridae
Acyrthosiphon pisum
010602 entomology
030104 developmental biology
Biological Control Agents
Insect Science
Horizontal gene transfer
Laboratory of Genetics
Female
Original Article
PEST analysis
Subjects
Details
- ISSN :
- 13652583 and 09621075
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Insect Molecular Biology
- Accession number :
- edsair.doi.dedup.....47bc220c3146efdb00affe099ef3acb2