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Anti-tumor effects of a human VEGFR-2-based DNA vaccine in mouse models
- Source :
- Genetic Vaccines and Therapy
- Publisher :
- Springer Nature
-
Abstract
- BackgroundVascular endothelial growth factor (VEGF) and its receptor, VEGFR-2 (Flk-1/KDR), play a key role in tumor angiogenesis. Blocking the VEGF-VEGFR-2 pathway may inhibit tumor growth. Here, we used human VEGFR-2 as a model antigen to explore the feasibility of immunotherapy with a plasmid DNA vaccine based on a xenogeneic homologue of this receptor.MethodsThe protective effects and therapeutic anti-tumor immunity mediated by the DNA vaccine were investigated in mouse models. Anti-angiogenesis effects were detected by immunohistochemical staining and the alginate-encapsulate tumor cell assay. The mechanism of action of the DNA vaccine was primarily explored by detection of auto-antibodies and CTL activity.ResultsThe DNA vaccine elicited a strong, protective and therapeutic anti-tumor immunity through an anti-angiogenesis mechanism in mouse models, mediated by the stimulation of an antigen-specific response against mFlk-1.ConclusionOur study shows that a DNA vaccine based on a xenogeneic homologue plasmid DNA induced autoimmunity against VEGFR-2, resulting in inhibition of tumor growth. Such vaccines may be clinically relevant for cancer immunotherapy.
- Subjects :
- medicine.medical_treatment
Genetic enhancement
Research
Immunology
Immunotherapy
Biology
DNA vaccination
Vascular endothelial growth factor
chemistry.chemical_compound
CTL
Mechanism of action
chemistry
Cancer immunotherapy
Immunity
medicine
Immunology and Allergy
Molecular Medicine
medicine.symptom
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 14790556
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Genetic Vaccines and Therapy
- Accession number :
- edsair.doi.dedup.....47d1de8b49be626d43d769051fc6d7d0
- Full Text :
- https://doi.org/10.1186/1479-0556-7-10