Supplementary Figure 1 from Contrary Regulation of Bladder Cancer Cell Proliferation and Invasion by Dexamethasone-Mediated Glucocorticoid Receptor Signals

PDF file - 5939K, The expression of GR in human BC. A, GR mRNA expression was analyzed in UMUC3, TCC-SUP, J82, and 5637 cells by RT-PCR, and transcripts (163 bp) were separated on an agarose gel. PCR products derived from GAPDH mRNA (71 bp) served as an internal control. B, Cell extracts from UMUC3, TCC-SUP, J82, and 5637 were analyzed on western blotting, using an antibody to GR (95 kDa, with a second band at 90 kDa). β-Actin protein (43 kDa) served as an internal control. C, Immunohistochemistry (IHC) of GR in bladder TMA, including benign urothelium, primary urothelial carcinoma, and lymph node (LN) metastasis. The Kaplan-Meier analysis was performed to assess progression-free survival in patients with BC, according to the levels of GR expression. Comparison was made by log-rank test. Tumor progression was defined as development of local recurrence or metastatic tumor.