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XMAP215 promotes microtubule-F-actin interactions to regulate growth cone microtubules during axon guidance
- Source :
- J Cell Sci
- Publication Year :
- 2019
- Publisher :
- The Company of Biologists, 2019.
-
Abstract
- It has long been established that neuronal growth cone navigation depends on changes in microtubule (MT) and F-actin architecture downstream of guidance cues. However, the mechanisms by which MTs and F-actin are dually coordinated remain a fundamentally unresolved question. Here, we report that the well-characterized MT polymerase, XMAP215 (also known as CKAP5), plays an important role in mediating MT–F-actin interaction within the growth cone. We demonstrate that XMAP215 regulates MT–F-actin alignment through its N-terminal TOG 1–5 domains. Additionally, we show that XMAP215 directly binds to F-actin in vitro and co-localizes with F-actin in the growth cone periphery. We also find that XMAP215 is required for regulation of growth cone morphology and response to the guidance cue, Ephrin A5. Our findings provide the first strong evidence that XMAP215 coordinates MT and F-actin interaction in vivo. We suggest a model in which XMAP215 regulates MT extension along F-actin bundles into the growth cone periphery and that these interactions may be important to control cytoskeletal dynamics downstream of guidance cues. This article has an associated First Person interview with the first author of the paper.
- Subjects :
- CKAP5
Growth Cones
Xenopus
macromolecular substances
Xenopus Proteins
Biology
Microtubules
Xenopus laevis
03 medical and health sciences
0302 clinical medicine
Microtubule
Animals
Cytoskeleton
Growth cone
Actin
030304 developmental biology
0303 health sciences
Cell Biology
biology.organism_classification
Ephrin-A5
Actins
Axons
Axon Guidance
Cell biology
Actin Cytoskeleton
Axon guidance
Ephrin A5
Microtubule-Associated Proteins
030217 neurology & neurosurgery
Research Article
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....481b06ec873007340df32b05c183944f