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Heterogeneity of anti-beta2-glycoprotein I antibodies. A factor of variability in test results
- Source :
- Thrombosis and haemostasis. 93(1)
- Publication Year :
- 2005
-
Abstract
- SummaryThe aim of this study was to evaluate the heterogeneity of IgG-anti- β2 -glycoprotein I antibodies (IgG-aβ2 GPI) as regarding their reactivity pattern against different sources of human β2 GPI,their avidity and their association with clinical events of antiphospholipid syndrome (APS).Three thousand six hundred and eightyfour consecutive patient sera were routinely tested for IgG-a β2 GPI over 1 year using an in-house ELISA with 2 different commercial preparations of human purified β2GPI. Of the 340 sera found positive, all those clinically documented were included in this study; 61 were positive with only one preparation (S1) and 59 with both (S2).The results of ELISA were confirmed by Western blot. Heterogeneity was stressed by testing sera with a human recombinant protein and 3 β2 GPI-related peptides. No contribution of glycosylation in the binding to β2GPI was found.The avidity indices for each protein were significantly higher in S1 than in S2 (p = 0.0021). S2 were more associated with antiphospholipid antibodies than S1 (75% versus 21% ; p
- Subjects :
- Glycosylation
Antibody Affinity
Enzyme-Linked Immunosorbent Assay
Cross Reactions
medicine.disease_cause
Autoimmunity
law.invention
Western blot
Antiphospholipid syndrome
law
Medicine
Humans
Avidity
Autoantibodies
Glycoproteins
Autoimmune disease
biology
medicine.diagnostic_test
business.industry
Autoantibody
Reproducibility of Results
Hematology
medicine.disease
Antiphospholipid Syndrome
beta 2-Glycoprotein I
Immunology
biology.protein
Recombinant DNA
Antibody
business
Antibody Diversity
Subjects
Details
- ISSN :
- 03406245
- Volume :
- 93
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Thrombosis and haemostasis
- Accession number :
- edsair.doi.dedup.....483e3d333b8dea5f5d6edff7b4b787a5