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Blocking effect of intraperitoneal injection of phenylalanine on high-threshold calcium currents in rat hippocampal neurones

Authors :
Galina Skibo
Anatoly E. Martynyuk
S.V. Savina
Source :
Brain Research. 552:228-231
Publication Year :
1991
Publisher :
Elsevier BV, 1991.

Abstract

Calcium currents were recorded in cultured (5–7 days) hippocampal neurones isolated from one-day-old rats. The animals obtained intraperitoneal injections of l -phenylalanine which induces in the brain biochemical changes characteristic of phenylketonuria. It has been found that the amplitude of the low-threshold calcium current in l -phenylalanine-affected neurones was not appreciably changed compared with that in neurones from control (non-injected) animals. However, the amplitude of the high-threshold calcium current was essentially decreased. Its relative amplitude at Vt = +20 mV became 40 ± 30% as contrasted to 416 ± 130% in neurones from control animals (the amplitude of the calcium currents at Vt = −10 mV taken as 100%). The decrease remained during the whole time of culturing. Addition of l -tyrosine to the cultivation medium (50 μM) restored the high-voltage calcium current, its relative amplitude reaching 280 ± 57%. The data are discussed in conjunction with the previously obtained results about antagonistic modulatory action of tyrosine and phenylalanine on the functioning of high-threshold calcium channels and possible mechanisms of brain dysfunction during phenylketonuria.

Details

ISSN :
00068993
Volume :
552
Database :
OpenAIRE
Journal :
Brain Research
Accession number :
edsair.doi.dedup.....487a4866dfa406bf743bd37a3eaa55d3
Full Text :
https://doi.org/10.1016/0006-8993(91)90086-b