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Blocking effect of intraperitoneal injection of phenylalanine on high-threshold calcium currents in rat hippocampal neurones
- Source :
- Brain Research. 552:228-231
- Publication Year :
- 1991
- Publisher :
- Elsevier BV, 1991.
-
Abstract
- Calcium currents were recorded in cultured (5–7 days) hippocampal neurones isolated from one-day-old rats. The animals obtained intraperitoneal injections of l -phenylalanine which induces in the brain biochemical changes characteristic of phenylketonuria. It has been found that the amplitude of the low-threshold calcium current in l -phenylalanine-affected neurones was not appreciably changed compared with that in neurones from control (non-injected) animals. However, the amplitude of the high-threshold calcium current was essentially decreased. Its relative amplitude at Vt = +20 mV became 40 ± 30% as contrasted to 416 ± 130% in neurones from control animals (the amplitude of the calcium currents at Vt = −10 mV taken as 100%). The decrease remained during the whole time of culturing. Addition of l -tyrosine to the cultivation medium (50 μM) restored the high-voltage calcium current, its relative amplitude reaching 280 ± 57%. The data are discussed in conjunction with the previously obtained results about antagonistic modulatory action of tyrosine and phenylalanine on the functioning of high-threshold calcium channels and possible mechanisms of brain dysfunction during phenylketonuria.
- Subjects :
- medicine.medical_specialty
Phenylalanine
medicine.medical_treatment
Intraperitoneal injection
Central nervous system
chemistry.chemical_element
Calcium
Hippocampal formation
Hippocampus
Membrane Potentials
Reference Values
Internal medicine
medicine
Animals
Tyrosine
Molecular Biology
Cells, Cultured
Neurons
Voltage-dependent calcium channel
Chemistry
General Neuroscience
Rats
Kinetics
Electrophysiology
medicine.anatomical_structure
Endocrinology
Animals, Newborn
Calcium Channels
Neurology (clinical)
Injections, Intraperitoneal
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 552
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....487a4866dfa406bf743bd37a3eaa55d3
- Full Text :
- https://doi.org/10.1016/0006-8993(91)90086-b