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Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus
- Source :
- Journal of General Virology. 89:2315-2330
- Publication Year :
- 2008
- Publisher :
- Microbiology Society, 2008.
-
Abstract
- The complete genome of the Maruca vitrata nucleopolyhedrovirus (MaviNPV) isolated from the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), was sequenced. It was found to be 111 953 bp in length, with an overall 39 % G+C content, and contained 126 open reading frames (ORFs) encoding predicted proteins of over 50 aa. The gene content and gene order of MaviNPV have the highest similarity to those of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and their shared homologous genes are 100 % collinear. In fact, MaviNPV seems to be a mini-AcMNPV that is native to Taiwan and possesses a smaller genome with fewer auxiliary genes than the AcMNPV type species. Except for one ORF (Mv74), all of the MaviNPV ORFs have homologues in the AcMNPV genome. MaviNPV is the first lepidopteran-specific baculovirus to lack homologues of vfgf and odv-e66. In addition, MaviNPV lacks the baculovirus repeat ORF (bro) gene that corresponds to AcMNPV ORF2. Five homologous regions (hrs) were located within the MaviNPV genome, and these contained a total of 44 imperfect palindromes. Phylogenetic analysis of the whole genome revealed that MaviNPV was separated from the common ancestor of AcMNPV and Bombyx mori nucleopolyhedrovirus before these two viral species diverged from each other. Moreover, replication of MaviNPV in several cell lines and an egfp–MaviNPV infection assay revealed that IPLB-LD-652Y cells are only partially permissive to MaviNPV, which supports our conclusion that MaviNPV is a distinct species of the group I lepidopteran NPVs.
- Subjects :
- DNA Replication
Baculoviridae
viruses
Molecular Sequence Data
Genome, Viral
Moths
Genome
Cell Line
Open Reading Frames
Viral Proteins
Species Specificity
Virology
Animals
ORFS
Gene
Phylogeny
DNA Primers
Viral Structural Proteins
Base Composition
Base Sequence
biology
Phylogenetic tree
fungi
biology.organism_classification
Nucleopolyhedroviruses
Autographa californica
Open reading frame
Maruca vitrata
DNA, Viral
Host-Pathogen Interactions
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....48bf67a286d70990c05012dcf11b9690