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Membrane Protein Production in Lactococcus lactis for Functional Studies
- Source :
- Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, Mus-Veteau Isabelle. Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, 1432, Humana Press, New York, NY., pp.79-101, 2016, Methods in Molecular Biology, 978-1-4939-3637-3. ⟨10.1007/978-1-4939-3637-3_6⟩, Mus-Veteau Isabelle. Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, 1432, Humana Press, New York, NY., pp.79-101, 2016, 978-1-4939-3637-3. ⟨10.1007/978-1-4939-3637-3_6⟩, Methods in Molecular Biology, Mus-Veteau, I. Methods in Molecular Biology, 1432, Springer, New-York, USA, pp.79-101, 2016, Heterologous Expression of Membrane Proteins, 978-1-4939-3637-3, Mus-Veteau Isabelle. Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, 1432, Humana Press, pp.79-101, 2016, Methods in Molecular Biology, 978-1-4939-3637-3. ⟨10.1007/978-1-4939-3637-3_6⟩, Methods in Molecular Biology ISBN: 9781493936359
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- International audience; Due to their unique properties, expression and study of membrane proteins in heterologous systems remains difficult. Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis, traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis.
- Subjects :
- 0301 basic medicine
Heterologous
Expression
law.invention
03 medical and health sciences
Transport assays
law
Gene expression
Membrane proteins
Protocol
Functional studies
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]
[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology
ComputingMilieux_MISCELLANEOUS
030102 biochemistry & molecular biology
biology
[ SDV ] Life Sciences [q-bio]
Lactococcus lactis
food and beverages
Protein engineering
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
030104 developmental biology
Biochemistry
Membrane protein
Recombinant DNA
Protein overexpression
Bacteria
Subjects
Details
- Language :
- English
- ISBN :
- 978-1-4939-3637-3
978-1-4939-3635-9 - ISBNs :
- 9781493936373 and 9781493936359
- Database :
- OpenAIRE
- Journal :
- Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, Mus-Veteau Isabelle. Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, 1432, Humana Press, New York, NY., pp.79-101, 2016, Methods in Molecular Biology, 978-1-4939-3637-3. ⟨10.1007/978-1-4939-3637-3_6⟩, Mus-Veteau Isabelle. Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, 1432, Humana Press, New York, NY., pp.79-101, 2016, 978-1-4939-3637-3. ⟨10.1007/978-1-4939-3637-3_6⟩, Methods in Molecular Biology, Mus-Veteau, I. Methods in Molecular Biology, 1432, Springer, New-York, USA, pp.79-101, 2016, Heterologous Expression of Membrane Proteins, 978-1-4939-3637-3, Mus-Veteau Isabelle. Heterologous Expression of Membrane Proteins. Methods in Molecular Biology, 1432, Humana Press, pp.79-101, 2016, Methods in Molecular Biology, 978-1-4939-3637-3. ⟨10.1007/978-1-4939-3637-3_6⟩, Methods in Molecular Biology ISBN: 9781493936359
- Accession number :
- edsair.doi.dedup.....491ffd03a054351dd8a58e678cb9a702
- Full Text :
- https://doi.org/10.1007/978-1-4939-3637-3_6⟩