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miR‑514a‑3p functions as a tumor suppressor in renal cell carcinoma
- Source :
- Oncology Letters.
- Publication Year :
- 2017
- Publisher :
- Spandidos Publications, 2017.
-
Abstract
- Renal cell carcinoma (RCC) is the most common type of kidney cancer, and the prognosis of metastatic RCC remains poor with a high rate of recurrence and mortality. A previous study has revealed that microRNA (miRNA), which negatively regulates protein expression, serves a role of oncogene or tumor suppressor. The aim of the present study was to investigate the expression and function of miR-514a-3p in RCC. To detect the expression of miR-514a-3p in 32 paired RCC tissues, quantitative polymerase chain reaction (qPCR) was performed. The function of miR-514a-3p in the proliferation, mobility and apoptosis of RCC cells (786-O and ACHN) was assessed by MTT, CCK-8, cell scratch, Transwell, Hoechst 33342 staining and flow cytometry assay. The results of qPCR revealed that miR-514a-3p was significantly downregulated in RCC tissues compared with adjacent normal tissues. Upregulation of miR-514a-3p by transfection of mimics suppressed RCC cell proliferation, migration and invasion, and induced cell apoptosis. The results revealed that miR-514a-3p was significantly downregulated in RCC and may serve a role as tumor suppressor in RCC. Further studies are required, focusing on the possibility of using miR-514a-3p as a biomarker for RCC as well as the pathway of miR-514a-3p in RCC.
- Subjects :
- 0301 basic medicine
Cancer Research
Cell
Biology
urologic and male genital diseases
medicine.disease_cause
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Renal cell carcinoma
microRNA
medicine
neoplasms
Oncogene
medicine.diagnostic_test
Cell growth
Articles
Cell cycle
medicine.disease
female genital diseases and pregnancy complications
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Cancer research
Carcinogenesis
Subjects
Details
- ISSN :
- 17921082 and 17921074
- Database :
- OpenAIRE
- Journal :
- Oncology Letters
- Accession number :
- edsair.doi.dedup.....492cb08656ac07c330268eb31a7a5ec4