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Bioaccumulation and biotransformation of pyrene and 1-hydroxypyrene by the marine whelkBuccinum undatum
- Source :
- Environmental Toxicology and Chemistry. 29:779-788
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- The fates of a phenolic contaminant and its hydrocarbon precursor have rarely been compared, especially in an invertebrate species. Two groups of Buccinum undatum were exposed to equimolar amounts of pyrene and 1-hydroxypyrene over 15 d through their diets. Tissue extracts from the muscle and visceral mass were analyzed by liquid chromatography with fluorescence and mass spectrometry detection. Nine biotransformation products were detected in animals from both exposures. These included 1-hydroxy- pyrene, pyrene-1-sulfate, pyrene-1-glucuronide, pyrene glucose sulfate, two isomers each of pyrenediol sulfate and pyrenediol disulfate, and one isomer of pyrenediol glucuronide sulfate. These compounds represent a more complex metabolic pathway for pyrene than is typically reported. Diconjugated metabolites were as important in animals exposed to pyrene as in those exposed to 1-hydroxypyrene. Biotransformation products represented >90% of the material detected in the animals and highlight the importance of analyzing metabolites when assessing exposure. A mean of only 2 to 3% of the body burden was present in muscle compared with the visceral mass of both groups. The analytical methods were sufficiently sensitive to detect biotransformation products both in laboratory control whelks and in those sampled offshore. The tissue distribution of ( 14 C)pyrene was also studied by autoradiography. Radioactivity was present primarily in the digestive and excretory system of the whelks and not in the gonads or muscle tissue. Environ. Toxicol. Chem. 2010;29:779-788. # 2009 SETAC Keywords—Dietary uptake Polycyclic aromatic hydrocarbons Metabolite Phenolic Invertebrate
- Subjects :
- Muscle tissue
Health, Toxicology and Mutagenesis
Metabolite
Gastropoda
Buccinum undatum
Dietary uptake
Fluorescence
Mass Spectrometry
chemistry.chemical_compound
Biotransformation
medicine
Animals
Environmental Chemistry
Tissue Distribution
Invertebrate
Sulfate
Chromatography, High Pressure Liquid
Pyrenes
biology
biology.organism_classification
Polycyclic aromatic hydrocarbons
medicine.anatomical_structure
Phenolic
chemistry
Bioaccumulation
Environmental chemistry
Pyrene
Glucuronide
Water Pollutants, Chemical
Subjects
Details
- ISSN :
- 15528618 and 07307268
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Environmental Toxicology and Chemistry
- Accession number :
- edsair.doi.dedup.....495df5d72e11b1fb8138408e596561e4