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Two-dimensional crystallization of membrane proteins: the lipid layer strategy
- Source :
- FEBS Letters. 504:187-193
- Publication Year :
- 2001
- Publisher :
- Wiley, 2001.
-
Abstract
- Due to the difficulty to crystallize membrane proteins, there is a considerable interest to intensify research topics aimed at developing new methods of crystallization. In this context, the lipid layer crystallization at the air/water interface, used so far for soluble proteins, has been recently adapted successfully to produce two-dimensional (2D) crystals of membrane proteins, amenable to structural analysis by electron crystallography. Besides to represent a new alternative strategy, this approach gains the advantage to decrease significantly the amount of material needed in incubation trials, thus opening the field of crystallization to those membrane proteins difficult to surexpress and/or purify. The systematic studies that have been performed on different classes of membrane proteins are reviewed and the physico-chemical processes that lead to the production of 2D crystals are addressed. The different drawbacks, advantages and perspectives of this new strategy for providing structural information on membrane proteins are discussed.
- Subjects :
- Protein Conformation
Lipid Bilayers
Biophysics
Context (language use)
Crystallography, X-Ray
Models, Biological
Biochemistry
law.invention
Structural Biology
law
Genetics
Animals
Crystallization
Lipid bilayer
Molecular Biology
Micelles
Microscopy
Chemistry
Electron crystallography
Air
Cell Membrane
Lipid layer
Water
Cell Biology
Lipid Metabolism
Two-dimensional crystallization
Membrane protein
Lipid ligand
Alternative strategy
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 504
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....495e97d3d923b9ee0f0c3e22f0edf983
- Full Text :
- https://doi.org/10.1016/s0014-5793(01)02748-x