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Thaumatin-like proteins and a cysteine protease inhibitor secreted by the pine wood nematode Bursaphelenchus xylophilus induce cell death in Nicotiana benthamiana
- Source :
- PLoS ONE, PLoS ONE, Vol 15, Iss 10, p e0241613 (2020)
- Publication Year :
- 2020
- Publisher :
- Public Library of Science, 2020.
-
Abstract
- Pine wilt disease (PWD) is an infectious disease of pines that typically kills affected trees. The causal pathogen of PWD is the pine wood nematode (PWN), Bursaphelenchus xylophilus. Understanding of the disease has advanced in recent years through the use of a highly sensitive proteomics procedure and whole genome sequence analysis; in combination, these approaches have enabled identification of proteins secreted by PWNs. However, the roles of these proteins during the onset of parasitism have not yet been elucidated. In this study, we used a leaf-disk assay based on transient overexpression in Nicotiana benthamiana to allow functional screening of 10 candidate pathogenic proteins secreted by PWNs. These proteins were selected based on previous secretome and RNA-seq analyses. We found that five molecules induced significant cell death in tobacco plants relative to a GFP-only control. Three of these proteins (Bx-TH1, Bx-TH2, and Bx-CPI) may have a role in molecular mimicry and likely make important contributions to inducing hypersensitive responses in host plants.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Tylenchida
Science
Nicotiana benthamiana
Bursaphelenchus xylophilus
Cysteine Proteinase Inhibitors
Proteomics
01 natural sciences
Microbiology
Host-Parasite Interactions
03 medical and health sciences
Tobacco
Animals
Pathogen
Wilt disease
Nicotiana
Plant Diseases
Multidisciplinary
biology
Cell Death
fungi
food and beverages
Helminth Proteins
biology.organism_classification
Cysteine protease
030104 developmental biology
Thaumatin
Medicine
010606 plant biology & botany
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....49672ee6230c9cbfe8e1173c09c9fcd0