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Interactions between Transport Protein Particle (TRAPP) complexes and Rab GTPases in Arabidopsis
- Source :
- Plant J. 100, 279-297 (2019)
- Publication Year :
- 2019
-
Abstract
- Transport Protein Particle II (TRAPPII) is essential for exocytosis, endocytosis, protein sorting and cytokinesis. In spite of a considerable understanding of its biological role, little information is known about Arabidopsis TRAPPII complex topology and molecular function. In this study, independent proteomic approaches initiated with TRAPP components or Rab-A GTPase variants converge on the TRAPPII complex. We show that the Arabidopsis genome encodes the full complement of 13 TRAPPC subunits, including four previously unidentified components. A dimerization model is proposed to account for binary interactions between TRAPPII subunits. Preferential binding to dominant negative (GDP-bound) versus wild-type or constitutively active (GTP-bound) RAB-A2a variants discriminates between TRAPPII and TRAPPIII subunits and shows that Arabidopsis complexes differ from yeast but resemble metazoan TRAPP complexes. Analyzes of Rab-A mutant variants in trappii backgrounds provide genetic evidence that TRAPPII functions upstream of RAB-A2a, allowing us to propose that TRAPPII is likely to behave as a guanine nucleotide exchange factor (GEF) for the RAB-A2a GTPase. GEFs catalyze exchange of GDP for GTP; the GTP-bound, activated, Rab then recruits a diverse local network of Rab effectors to specify membrane identity in subsequent vesicle fusion events. Understanding GEF-Rab interactions will be crucial to unravel the co-ordination of plant membrane traffic.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Proteomics
Proteome
Arabidopsis
Vesicular Transport Proteins
Plant Science
GTPase
medicine.disease_cause
01 natural sciences
Models, Biological
Exocytosis
03 medical and health sciences
Protein targeting
Genetics
medicine
Arabidopsis thaliana
Guanine Nucleotide Exchange Factors
Cytokinesis
Secretory Pathway
biology
Arabidopsis Thaliana
Rab Gtpases
Tethering Complexes
Arabidopsis Proteins
fungi
Cell Biology
biology.organism_classification
Cell biology
Protein Transport
030104 developmental biology
rab GTP-Binding Proteins
Mutation
Rab
Guanine nucleotide exchange factor
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1365313X
- Volume :
- 100
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Plant journal : for cell and molecular biologyReferences
- Accession number :
- edsair.doi.dedup.....4989c49c9cf3c84955e9ff0e4f6411a8