Application of material in filter paper chromatography

IN order to obtain a good separation in filter paper chromatography, the size of the spot of liquid applied to the paper must be small, which often necessitates repeated, time-consuming applications of material. Methods have been evolved with the purpose of having spots or a. band laid down automatically by a burette1,2. There still seems to be a need, however, for a technique by which a larger volume of fluid can be deposited quickly and simply, yet permitting small-sized bands or spots for good separation.