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Specific cellular and humoral immune response in Balbc mice immunised with an expression genomic library of Trypanosoma cruzi

Specific cellular and humoral immune response in Balbc mice immunised with an expression genomic library of Trypanosoma cruzi

Authors :
J. F. Infante
Luis Izquierdo
Alberto Fachado
Maria E. Sarmiento
Luis Fonte
Armando Acosta
G. Sierra
Esteban Alberti
Carlos M. Finlay
Teresita Vidal
Carlos Hidalgo
Source :
Vaccine. 16:608-612
Publication Year :
1998
Publisher :
Elsevier BV, 1998.

Abstract

An expression genomic library of Trypanosoma cruzi (T. cruzi) constructed using pcDNA3 plasmid was used for the immunisation (25 ug) of Balblc mice. Expression of T. cruzi antigens in the muscle of inoculated mice was detected by indirect immunofluorescence 7 days after immunisation. Specific IgG antibodies were significatively increased (P ~0.05) in animals that were reimmunised with 50 ,ug of the genomic library. An antigen specific lymphoproliferative response was detected in one animal of 0 1998 Elsevier Science Ltd. All the group inoculated with one dose of the library rights reserved

Details

ISSN :
0264410X
Volume :
16
Database :
OpenAIRE
Journal :
Vaccine
Accession number :
edsair.doi.dedup.....49b23d12dd85d1d4d4cd7b315ae12587
Full Text :
https://doi.org/10.1016/s0264-410x(97)00228-4