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Specific cellular and humoral immune response in Balbc mice immunised with an expression genomic library of Trypanosoma cruzi
Specific cellular and humoral immune response in Balbc mice immunised with an expression genomic library of Trypanosoma cruzi
- Source :
- Vaccine. 16:608-612
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- An expression genomic library of Trypanosoma cruzi (T. cruzi) constructed using pcDNA3 plasmid was used for the immunisation (25 ug) of Balblc mice. Expression of T. cruzi antigens in the muscle of inoculated mice was detected by indirect immunofluorescence 7 days after immunisation. Specific IgG antibodies were significatively increased (P ~0.05) in animals that were reimmunised with 50 ,ug of the genomic library. An antigen specific lymphoproliferative response was detected in one animal of 0 1998 Elsevier Science Ltd. All the group inoculated with one dose of the library rights reserved
- Subjects :
- Protozoan Vaccines
Cellular immunity
Trypanosoma cruzi
Enzyme-Linked Immunosorbent Assay
Lymphocyte Activation
Mice
Immune system
Antigen
Antibody Specificity
parasitic diseases
Vaccines, DNA
Animals
Immunity, Cellular
Mice, Inbred BALB C
General Veterinary
General Immunology and Microbiology
biology
Immunogenicity
Public Health, Environmental and Occupational Health
DNA, Protozoan
biology.organism_classification
Virology
Infectious Diseases
Immunoglobulin G
Antibody Formation
Humoral immunity
biology.protein
Molecular Medicine
Female
Antibody
Genome, Protozoan
Lymphoproliferative response
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....49b23d12dd85d1d4d4cd7b315ae12587
- Full Text :
- https://doi.org/10.1016/s0264-410x(97)00228-4