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Endurance Exercise Accelerates Myocardial Tissue Oxygenation Recovery and Reduces Ischemia Reperfusion Injury in Mice
- Source :
- PLoS ONE, Vol 9, Iss 12, p e114205 (2014), PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- Exercise training offers cardioprotection against ischemia and reperfusion (I/R) injury. However, few essential signals have been identified to underscore the protection from injury. In the present study, we hypothesized that exercise-induced acceleration of myocardial tissue oxygenation recovery contributes to this protection. C57BL/6 mice (4 weeks old) were trained on treadmills for 45 min/day at a treading rate of 15 m/min for 8 weeks. At the end of 8-week exercise training, mice underwent 30-min left anterior descending coronary artery occlusion followed by 60-min or 24-h reperfusion. Electron paramagnetic resonance oximetry was performed to measure myocardial tissue oxygenation. Western immunoblotting analyses, gene transfection, and myography were examined. The oximetry study demonstrated that exercise markedly shortened myocardial tissue oxygenation recovery time following reperfusion. Exercise training up-regulated Kir6.1 protein expression (a subunit of ATP-sensitive K(+)channel on vascular smooth muscle cells, VSMC sarc-K(ATP)) and protected the heart from I/R injury. In vivo gene transfer of dominant negative Kir6.1AAA prolonged the recovery time and enlarged infarct size. In addition, transfection of Kir6.1AAA increased the stiffness and reduced the relaxation capacity in the vasculature. Together, our study demonstrated that exercise training up-regulated Kir6.1, improved tissue oxygenation recovery, and protected the heart against I/R injury. This exercise-induced cardioprotective mechanism may provide a potential therapeutic intervention targeting VSMC sarc-K(ATP) channels and reperfusion recovery.
- Subjects :
- Pathology
Vascular smooth muscle
Myocardial Infarction
Gene Expression
Coronary Artery Disease
030204 cardiovascular system & hematology
Vascular Medicine
Muscle, Smooth, Vascular
Mice
0302 clinical medicine
KATP Channels
Biological Systems Engineering
Medicine and Health Sciences
Biomechanics
Cardioprotection
0303 health sciences
Multidisciplinary
Electrical impedance myography
Cardiovascular Diseases
Cardiology
Engineering and Technology
Medicine
Anatomy
Cardiomyopathies
Research Article
Biotechnology
medicine.medical_specialty
Science
Biophysics
Biomedical Engineering
Aortic Diseases
Ischemia
Bioengineering
Myocardial Reperfusion Injury
03 medical and health sciences
Oxygen Consumption
Endurance training
Physical Conditioning, Animal
Internal medicine
medicine
Animals
Humans
030304 developmental biology
business.industry
Acute Cardiovascular Problems
Myocardium
Biology and Life Sciences
Blood flow
Oxygenation
medicine.disease
Mice, Inbred C57BL
Cardiovascular Anatomy
business
Reperfusion injury
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....49b49cc5f2c7eb45801860a68793069d