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Sensitive Detection of Single-Nucleotide Polymorphisms by Solid Nanopores Integrated With DNA Probed Nanoparticles
- Source :
- Frontiers in Bioengineering and Biotechnology, Vol 9 (2021), Frontiers in Bioengineering and Biotechnology
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- Single-nucleotide polymorphisms (SNPs) are the abundant forms of genetic variations, which are closely associated with serious genetic and inherited diseases, even cancers. Here, a novel SNP detection assay has been developed for single-nucleotide discrimination by nanopore sensing platform with DNA probed Au nanoparticles as transport carriers. The SNP of p53 gene mutation in gastric cancer has been successfully detected in the femtomolar concentration by nanopore sensing. The robust biosensing strategy offers a way for solid nanopore sensors integrated with varied nanoparticles to achieve single-nucleotide distinction with high sensitivity and spatial resolution, which promises tremendous potential applications of nanopore sensing for early diagnosis and disease prevention in the near future.
- Subjects :
- solid nanopore
Histology
Biomedical Engineering
Nanoparticle
Bioengineering
Single-nucleotide polymorphism
Nanotechnology
02 engineering and technology
Gene mutation
010402 general chemistry
01 natural sciences
chemistry.chemical_compound
single nucleotide polymorphism
Hybridization probe
gastric cancer
Bioengineering and Biotechnology
Brief Research Report
021001 nanoscience & nanotechnology
0104 chemical sciences
Nanopore
DNA probe
chemistry
Disease prevention
0210 nano-technology
Biosensor
DNA
gold nanoparticle
TP248.13-248.65
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 22964185
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Frontiers in Bioengineering and Biotechnology
- Accession number :
- edsair.doi.dedup.....49c726dc6d845f4a32b021c521907df8