Back to Search
Start Over
Inhibitory effect of emodin on migration, invasion and metastasis of human breast cancer MDA-MB-231 cells in vitro and in vivo
- Source :
- Oncology Reports. 33:338-346
- Publication Year :
- 2014
- Publisher :
- Spandidos Publications, 2014.
-
Abstract
- In breast cancer, metastasis is the main reason for patient mortality. In the present study, we used breast cancer MDA-MB-231 cells and a mouse xenograft model to demonstrate the effect of emodin on the migration, invasion and metastasis of human breast cancer MDA-MB-231 cells and the related mechanisms. In vitro, wound healing and Transwell assays showed that emodin dose-dependently inhibited the migration and invasion of MDA-MB-231 cells. Enzyme-linked immunosorbent assay (ELISA) showed that emodin decreased the secretion of MMP-2 and MMP-9. Western blot analysis showed that emodin downregulated the expression levels of MMP-2, MMP-9, uPA and uPAR as well as p38 inhibitor SB203580 and ERK inhibitor PD980559, even though TIMP-1 and TIMP-2 were not obviously changed in the MDA-MB-231 cells. Furthermore, emodin inhibited the activity of p38 and ERK1/2 in the MDA-MB-231 cells. In vivo, emodin inhibited lung metastasis in mice bearing the breast cancer MDA-MB-231 xenografts with no obvious changes in body weight, liver and kidney functions. These results indicated that emodin inhibited the lung metastasis of human breast cancer in a mouse xenograft model, and inhibited the invasion of MDA-MB-231 cells associated with the downregulation of MMP-2, MMP-9, uPA and uPAR expression as well as decreased activity of p38 and ERK.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Emodin
Lung Neoplasms
Mice, Nude
Antineoplastic Agents
Breast Neoplasms
Biology
p38 Mitogen-Activated Protein Kinases
Metastasis
chemistry.chemical_compound
Breast cancer
Cell Movement
In vivo
Cell Line, Tumor
medicine
Animals
Humans
skin and connective tissue diseases
Dose-Response Relationship, Drug
Oncogene
Body Weight
Cancer
General Medicine
medicine.disease
Urokinase-Type Plasminogen Activator
Xenograft Model Antitumor Assays
Urokinase receptor
Matrix Metalloproteinase 9
Oncology
chemistry
Apoptosis
Cancer research
Matrix Metalloproteinase 2
Female
Subjects
Details
- ISSN :
- 17912431 and 1021335X
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....49cdcb2291689c5c65ce8fe0897b5b00