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Background-free imaging of chemical bonds by a simple and robust frequency-modulated stimulated Raman scattering microscopy
- Source :
- Optics express, vol 28, iss 10, Opt Express
- Publication Year :
- 2020
- Publisher :
- Optica Publishing Group, 2020.
-
Abstract
- Being able to image chemical bonds with high sensitivity and speed, stimulated Raman scattering (SRS) microscopy has made a major impact in biomedical optics. However, it is well known that the standard SRS microscopy suffers from various backgrounds, limiting the achievable contrast, quantification and sensitivity. While many frequency-modulation (FM) SRS schemes have been demonstrated to retrieve the sharp vibrational contrast, they often require customized laser systems and/or complicated laser pulse shaping or introduce additional noise, thereby hindering wide adoption. Herein we report a simple but robust strategy for FM-SRS microscopy based on a popular commercial laser system and regular optics. Harnessing self-phase modulation induced self-balanced spectral splitting of picosecond Stokes beam propagating in standard single-mode silica fibers, a high-performance FM-SRS system is constructed without introducing any additional signal noise. Our strategy enables adaptive spectral resolution for background-free SRS imaging of Raman modes with different linewidths. The generality of our method is demonstrated on a variety of Raman modes with effective suppressing of backgrounds including non-resonant cross phase modulation and electronic background from two-photon absorption or pump-probe process. As such, our method is promising to be adopted by the SRS microscopy community for background-free chemical imaging.
- Subjects :
- Chemical imaging
Femtosecond pulse shaping
Materials science
Physics::Optics
Bioengineering
Optical Physics
02 engineering and technology
01 natural sciences
Noise (electronics)
Article
law.invention
010309 optics
symbols.namesake
Optics
law
0103 physical sciences
Microscopy
Electrical and Electronic Engineering
Communications Technologies
business.industry
021001 nanoscience & nanotechnology
Laser
Pulse shaping
Atomic and Molecular Physics, and Optics
symbols
0210 nano-technology
business
Raman spectroscopy
Raman scattering
Subjects
Details
- ISSN :
- 10944087
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Optics Express
- Accession number :
- edsair.doi.dedup.....49d4b0c9a03e36d230e2f11b8d81cbb1
- Full Text :
- https://doi.org/10.1364/oe.391016