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Molecular characterization of Cryptosporidium spp. in pre-weaned kids in a dairy goat farm in western France
- Source :
- Veterinary Parasitology, Veterinary Parasitology, Elsevier, 2013, 192 (1-3), pp.268-272. ⟨10.1016/j.vetpar.2012.11.008⟩
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- A longitudinal study was undertaken to characterize the course of Cryptosporidium infection in a dairy goat farm located in western France. Two cohorts of twenty-five and fifteen animals, respectively, were sampled once a week from birth to weaning. Each individual fecal sample was screened using direct immunofluorescence (IFT) and if found positive, the Cryptosporidium species was identified using PCR analysis. Seventeen (68% [95% CI: 44–91]) animals were positive at least once during the first study and 14 (93% [95% CI: 80–100]) during the second, after IFT examination. In the first study, the age at first excretion was 17 days and the peak of excretion (mean arithmetic excretion: 22,700 oocysts per gram (opg) of feces) was recorded when kids were between 22 and 28 days old. For the second study, the age at first excretion was 10 days and the peak of excretion (mean arithmetic excretion: 3.4 × 10 6 opg) was recorded in animals aged between 10 and 14 days. Clinical signs were observed only in animals of the second cohort. DNA sequence analysis at the 18S ribosomal RNA locus was successful for 9 of the 27 IFT-positive samples in the first cohort and for 10 of the 34 positive isolates in the second cohort. All isolates were identified as Cryptosporidium xiaoi except one which was identified as Cryptosporidium parvum. Our results confirm that goat kids are hosts for C. parvum and C. xiaoi and that infection by C. xiaoi may be associated with mild clinical signs.
- Subjects :
- Diarrhea
Veterinary medicine
Neonatal diarrhea
Cryptosporidium infection
040301 veterinary sciences
[SDV]Life Sciences [q-bio]
Cryptosporidiosis
Cryptosporidium
DNA, Ribosomal
Polymerase Chain Reaction
Cohort Studies
0403 veterinary science
Excretion
Feces
03 medical and health sciences
Animal science
Prevalence
RNA, Ribosomal, 18S
medicine
Animals
Weaning
Cryptosporidium xiaoi
Fluorescent Antibody Technique, Indirect
C. parvum
Direct fluorescent antibody
030304 developmental biology
0303 health sciences
Goat Diseases
General Veterinary
biology
Goats
Oocysts
Sequence Analysis, DNA
04 agricultural and veterinary sciences
General Medicine
DNA, Protozoan
biology.organism_classification
medicine.disease
3. Good health
Dairying
Cryptosporidium parvum
Cohort
Parasitology
France
Goat kids
Subjects
Details
- ISSN :
- 03044017
- Volume :
- 192
- Database :
- OpenAIRE
- Journal :
- Veterinary Parasitology
- Accession number :
- edsair.doi.dedup.....49f5459d17b204562a22e29642e12f17