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Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia
- Source :
- Forensic Science International. 311:110293
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Death by mechanical asphyxia is one of the most difficult conclusions to make in forensic science, especially in corpses displaying slight or no trauma to the surface of the body. Therefore, death by mechanical asphyxia is difficult to prove in medico-legal practice. MicroRNAs (miRNAs) are a class of small, non-coding RNAs involved in the regulation of numerous physiological and pathological cellular processes. In the present study, we demonstrate that significantly increased expression of miR-3185 in cardiac tissues was detected among cases of mechanical asphyxia compared to case of craniocerebral injury, hemorrhagic shock, sudden cardiac death and poisoning. We observed no correlation between the expression of miR-3185 and postmortem interval, age or temperature. Further work indicated that CYP4A11 is a putative target gene of miR-3185 and expressed at a relatively low level in cardiac tissue specimens from cases of mechanical asphyxia compared with specimens from cases of craniocerebral injury, hemorrhagic shock, sudden cardiac death and poisoning. Our results suggest that the miRNA-3185/CYP4A11 axis is associated with mechanical asphyxia-induced death and may provide new insight into asphyxial death investigations.
- Subjects :
- Adult
Forensic Genetics
Male
Pathology
medicine.medical_specialty
Adolescent
Craniocerebral injury
Shock, Hemorrhagic
Real-Time Polymerase Chain Reaction
01 natural sciences
Pathology and Forensic Medicine
Sudden cardiac death
Asphyxia
Young Adult
03 medical and health sciences
0302 clinical medicine
microRNA
medicine
Craniocerebral Trauma
Humans
RNA, Messenger
030216 legal & forensic medicine
Child
Forensic Pathology
Pathological
Aged
Aged, 80 and over
business.industry
Myocardium
Poisoning
010401 analytical chemistry
Middle Aged
medicine.disease
0104 chemical sciences
MicroRNAs
Death, Sudden, Cardiac
Child, Preschool
Postmortem Changes
Hemorrhagic shock
Biomarker (medicine)
Female
Cytochrome P-450 CYP4A
medicine.symptom
business
Law
Biomarkers
CYP4A11
Subjects
Details
- ISSN :
- 03790738
- Volume :
- 311
- Database :
- OpenAIRE
- Journal :
- Forensic Science International
- Accession number :
- edsair.doi.dedup.....49f929200ac1890fe218d99b20a2db25